The adverse effects of high glucose on stem cell therapy have been suggested through different mechanisms include, induces adipogenic differentiation of muscle-derived stem cells, 50 has an effect in stimulating mouse embryonic stem cell proliferation through the Ca 2+ /PKC, MAPKs, and the AT1 receptor, 49 insulin and estradiol are able to ...

High glucose (HG) concentrations impair cellular functions and induce apoptosis. Exposition of mesenchymal stem cells (MSC) to HG was reported to reduce colony forming activity and induce premature senescence. We characterized the effects of HG on human MSC in vitro using telomerase-immortalized MSC (hMSC-TERT) and primary MSC (hMSC).

MSCs cultured in medium containing high glucose (HG) concentrations exhibit premature senescence, genomic instability and telomere alterations (17–19) however, the molecular promotive mechanisms of HG in stem cell aging remain to be elucidated.

The present study reveals that during neural stem cell differentiation, both high glucose and H 2 O 2 induce ER stress markers, BiP and CHOP, leading to UPR activation manifested by increased phosphorylation of IRE1α and PERK. The ER stress inhibitor, 4-PBA, blocks the inhibitory effect of high glucose and H 2 O 2 on

High glucose (HG) culture conditions in vitro and persistent exposure to hyperglycemia in diabetes patients are detrimental to stem cells, analogous to any other cell type in our body. It interferes with diverse signaling pathways, i.e. mammalian ...