2021年4月14日 · Our work identifies HP1a as an epigenetic regulator that is involved in establishing the global structure of the genome in the early embryo. In metazoans, fertilization …

2024年8月28日 · Selective siRNA knockdown (KD) of endogenous HP1α in wild type HeLa (HeLa KD) and the cell lines exogenously transfected with eGFP-HP1α, eGFP-HP1α I165E or HP1α …

2023年3月9日 · We find that high level of HP1γ is associated with low acetylation modification in the bortezomib-resistant myeloma cells using SILAC-based acetyl-proteomics assay, and …

The HP1-rescue construct reverses the structural effects of HP1-KD (reduced contact frequency between the pericentromeric regions, as well as inter-chromosome arm interactions and …

2014年3月12日 · Our results reveal that NDR kinase catalyses the hinge-specific phosphorylation of human HP1α during G2/M in vivo and this orchestrates accurate chromosome alignment …

2013年5月17日 · Heterochromatin protein 1 (HP1) is an epigenetic gene silencing protein that is regulated by lysine 9 methylation of histone H3. Most eukaryotes have at least three HP1 …

2021年5月13日 · Here, by combining chromosome conformation capture (Hi-C), chromatin immunoprecipitation with high-throughput sequencing (ChIP–seq), 3D DNA fluorescence in …

KD of heterochromatin factor HP1γ increases expression of unintegrated HIV-1 DNAs. (A) Transient siRNA-based KD was performed for the three HP1 isoforms: HP1α, HP1β, HP1γ. …

We propose that the totipotent stem cell state was shaped by the need to resist transposon activity over evolutionary timescales. ... Therefore, dMes-4/NSD counteracts H3K27me3 …

Hi-C analysis of HP1-KD embryos at ZGA revealed major genome-wide changes in chromosome organization (Fig. 3a, Extended Data Fig. 3g, h); we found perturbed Rabl configuration with …