HS-5人骨髓基质细胞是一种源自一名30岁白人男性骨髓基质的细胞系，呈现典型的成纤维细胞形态。HS-5细胞系通过使用表达 HPV-16 E6/E7 蛋白的逆转录病毒载体（LXSN16E6E7）进行转化，转化过程中使用了聚乙烯吡咯烷酮（polybrene）来增强感染效率。

HS-5 is a cell with fibroblast morphology that was isolated from the stroma of a White, 30-year-old, male patient. This cell line was deposited by Fred Hutchinson Cancer Research Center and can be used for advanced immunology research.

Helicopter Sea Combat Squadron FIVE (HSC-5) (previously Helicopter Anti-Submarine Squadron FIVE (HS-5)), also known as the Nightdippers, is a helicopter squadron of the United States Navy based at Naval Station Norfolk operating the Sikorsky MH-60S Seahawk.

Established January 3rd, 1956, Nightdippers were scheduled to be redesignated Helicopter Sea Combat Squadron ( HSC-5 ) in 2009 when transition to the MH-60S Seahawk. 1956, I was assigned to HS1 in Aug55, deployed in October on the USS Tarawa with HOS3 Copters. In January 1956 about one half of HS1 transferred to HS5 when it was commissioned.

Derived from sampling site: Bone marrow stroma. Functionally distinct human marrow stromal cell lines immortalized by transduction with the human papilloma virus E6/E7 genes. Human marrow stromal cell lines which sustain hematopoiesis.

HS-5细胞是从一位30岁白人男性患者的骨髓基质中分离的具有成纤维形态的细胞，经过逆转录病毒载体LXSN16E6E7转化一共建立27个细胞系，依次命名为HS-1至HS-27。 该细胞系与HS-27A（SNL-623）来自于同一位患者。 HS-5表达G-CSF、GM-CSF、M-CSF、KL、巨噬细胞抑制蛋白1α、IL-1alpha、 IL-1beta、IL-1RA、IL-6、 IL-8、IL-11、 LIF基因，在无异源因子无血清培养基中共培养时支持造血祖细胞增殖。 消化传代细胞时吹打细胞注意尽量轻柔，不要产生过多 …

2020年11月20日 · 人骨髓基质细胞hs-5是指成体骨髓中的一类多能干细胞。 具有分化成骨细胞、软骨细胞、脂肪细胞和其他几种结缔组织细胞（如腱细胞）的潜能，亦可转分化成心肌细胞、骨骼肌细胞。

3) 轻轻吹打细胞，完全脱落后吸出至离心管中，1000rpm离心5-8min，弃去上清液，补 加1-2mL完全培养基后吹匀； 4) 按5-6mL/瓶补加完全培养基，将细胞悬液按1:2到1:3的比例分到新的含5-6mL完全

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hs-5 是一种具有成纤维细胞形态的细胞，从一名 30 岁的白人男性患者的基质中分离 出来。 人 30岁 骨髓；基质 贴壁细胞 成纤维细胞样 转化细胞系 dmem+10% fbs+1% p/s 1:2-1:4 2-3次/周 未知 冻存液：55%基础培养基+40% fbs+5% dmso温度：液氮 气相：空气，95%；co2，5% 温 …