In Vitro Assays for Hematopoietic Progenitor Cells. While transplantation assays are essential for evaluating the engraftment potential and hematopoietic reconstitution ability of HSPCs in vivo, culture assays have also emerged as valuable tools for assessing the proliferative and differentiation capacities of these cells.

Hematopoietic stem/progenitor cells (HSPCs) isolated from bone marrow have been successfully employed for 50 years in hematological transplantations. Currently, these cells are more frequently isolated from mobilized peripheral blood or umbilical cord blood. In this chapter, we overview several topi …

2020年12月10日 · Haematopoietic stem and progenitor cell (HSPC) gene therapy has emerged as an effective treatment modality for monogenic disorders of the blood system such as primary immunodeficiencies and β ...

Hematopoietic stem/progenitor cells (HSPC) have both unique and common responses following hemorrhage, injury, and sepsis. HSPCs from different lineages have a distinctive response to these ‘stress’ signals. Inflammation, via the production of ...

Abstract. Recent research has shed light on novel functions of hematopoietic stem and progenitor cells (HSPC). While they are critical for maintenance and replenishment of blood cells in the bone marrow, these cells are not limited to the bone marrow compartment and function beyond their role in hematopoiesis.

2019年6月3日 · Hematopoietic Stem/Progenitor cells (HSPCs) are endowed with the role of maintaining a diverse pool of blood cells throughout the human life. Despite recent efforts, the nature of the early cell ...

2019年4月11日 · The bone marrow (BM) microenvironment plays a crucial role in the maintenance and regeneration of hematopoietic stem (HSC) and progenitor cells (HSPC). In particular, the vascular niche is responsible for regulating HSC maintenance, differentiation, and migration of cells in and out of the BM. Damag …

To save time and reagents, and increase the accuracy of these analyses, it is preferable to perform immunophenotyping on purified CD34 + cells or lineage-depleted cells rather than on unfractionated bone marrow or blood, in which the overall HSPC frequency is very low. The need for clear immunophenotyping data is especially important when working with such rare cell …

Ongoing research on hematopoietic stem and progenitor cells (HSPCs) is directed toward the identification, isolation, and characterization of the primitive cell types that mediate rapid and/or sustained hematological recovery after cytoreductive therapy and transplantation.

Abstract. Hematopoietic stem cells (HSCs) reside in the bone marrow and are responsible for the life-time maintenance of the blood and bone marrow, achieved through their differentiation into the myriad cellular components and their ability to generate additional stem cells via self-renewal.