This cell line was derived from the pleural fluid of a patient with malignant mesothelioma. The patient had known exposure to crocidolite asbestos. JU77 is not tumourigenic in nude mice. Cells express cytokeratin and epithelial membrane antigen (EMA) but not mucin. Cells are spindle shaped with few vacuoles.

Parental cell: JU77; Organism: Human; Tissue: Lung; Disease: Cancer; Growth properties: Human mesothelioma cell line resistant to pegylated arginine deiminase (ADI-PEG20). Model: Cancer Model; Production details: JU77 cells were incubated with 10ug/ml ADI-PEG20 in RPMI-1640 media supplemented with 2% FBS and re-treated every week. After ...

This cell line was derived from the pleural fluid of a patient with malignant mesothelioma. The patient had known exposure to crocidolite asbestos. JU77 is not tumourigenic in nude mice. Cells express cytokeratin and epithelial membrane antigen (EMA) but not mucin. Cells are spindle shaped with few vacuoles. Cell Bank Australia reference number ...

Human mesothelioma cell line resistant to pegylated arginine deiminase (ADI-PEG20). RPMI-1640 media (Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS; Invitrogen), 100 U/ml penicillin, and 100ug/ml streptomycin at 37 o C/5% CO …

Establishment and characterization of five human malignant mesothelioma cell lines derived from pleural effusions. The nuclear deubiquitinase BAP1 is commonly inactivated by somatic mutations and 3p21.1 losses in malignant pleural mesothelioma. Secreted primary human malignant mesothelioma exosome signature reflects oncogenic cargo.

JU77 Lung Mesothelioma Cells JU77 cells were relatively easy to transfect ( Figure 6 ). Lipofectamine 3000 showed the highest transfection efficacy (normalized as 100%) in this study, followed by Lipofectamine 2000 (90.21%), but these two transfection reagents also displayed high cytotoxicity to JU77 cells (68.21% and 69.27% viability).

Cell line JU77, a previously well-characterised malignant mesothelioma cell line , was used as a positive control, and cells stained without primary antibody were taken as negative controls. Anchorage-independent Growth

【Complete Growth Medium完全培养基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Volumes are given for a 75 cm2 flask.

Cell Line Description: JU77 is a human mesothelioma cell line derived from a male patient. The patient had known exposure to crocidolite asbestos. The culture was developed

Part of: Next Generation Genetic Association studies (Next Gen) program cell lines. From: Becker, Lewis Charles; Johns Hopkins University School of Medicine; Baltimore; USA. Population: African American. Derived from site: In situ; Peripheral blood; UBERON= UBERON_0000178.