2023年5月7日 · Based on the sequences of kti1/3 mutant alleles in #5-26, we developed markers to co-select for these mutant alleles by using a gel-electrophoresis-free method. The kti1/3 mutant soybean line and associated selection markers will assist in accelerating the introduction of low TI trait into elite soybean cultivars in the future.

Q39091 · KTI1_ARATH. Protein. Kunitz trypsin inhibitor 1. Gene. KTI1. Status. UniProtKB reviewed (Swiss-Prot) Organism. Arabidopsis thaliana (Mouse-ear cress) Amino acids. 209 (go to sequence) Protein existence. Evidence at transcript level. Annotation score. 3/5. Entry. Variant viewer. Feature viewer. Genomic coordinates. Publications.

2008年5月1日 · Here, we show that a serine protease (Kunitz trypsin) inhibitor (KTI1) of Arabidopsis acts as a functional KTI when produced in bacteria and in planta. Expression of AtKTI1 is induced late in response to bacterial and fungal elicitors and to salicylic acid.

In this study, we aimed to (1) simultaneously knockout KTI1 and KTI3 genes in soybean cultivar Williams 82 via CRISPR/Cas9-mediated genome editing and (2) develop molecular markers associated with kti1 and kti3 mutant alleles that can be used for marker-assisted selection (MAS). We successfully recovered

arabidopsis thaliana kunitz trypsin inhibitor 1, arabidopsis thaliana kunitz trypsin inhibitor 4, atkti1, atkti4, kti1, kunitz trypsin inhibitor 1 Description Encodes a trypsin inhibitor involved in modulating programmed cell death in plant-pathogen interactions.

2021年5月10日 · 根据大豆基因组序列，扩增kti1 基因 atg 上游启动子序列 1 831 bp；生物信息学预测分析表明启动子中存在多种常出现在逆境胁迫诱导型启动子中的顺式调控元件，推测大豆 kti1 基因启动子可能是受低温、盐、干旱和 aba 诱导的诱导型启动子。

2023年10月28日 · Trypsin inhibitors (TIs) in soybean seeds reduce the availability of processed soybean foods and animal feed. This study aimed to evaluate the trypsin inhibitor activity (TIA) in 999 Korean soybean accessions and conduct molecular characterization of soybean accessions with low TIA. TIA was evaluated using colorimetric analysis through a substrate–enzyme reaction. The average TIA of the 999 ...

在这里，我们显示拟南芥的丝氨酸蛋白酶（Kunitz胰蛋白酶）抑制剂（KTI1）在细菌和植物中产生时起功能性KTI的作用。 AtKTI1的表达在响应细菌和真菌引发剂以及水杨酸后被诱导。

2023年5月8日 · Based on the sequences of kti1/3 mutant alleles in #5-26, we developed markers to co-select for these mutant alleles by using a gel-electrophoresis-free method. The kti1 / 3 mutant soybean line and associated selection markers will assist in accelerating the introduction of low TI trait into elite soybean cultivars in the future.

Three Kunitz trypsin inhibitor genes, designated as KTi1, KTi2, and KTi3, do not contain intervening sequences, and are expressed during embryogenesis and in the mature plant. The KTi1 and KTi2 genes have nearly identical nucleotide sequences, are expressed at different levels during embryogenesis, are represented in leaf, root, and stem mRNAs ...