The Lowry protein assay is a biochemical assay for determining the total level of protein in a solution. The total protein concentration is exhibited by a color change of the sample solution in proportion to protein concentration, which can then be measured using colorimetric techniques.

Under alkaline conditions the divalent copper ion forms a complex with peptide bonds in which it is reduced to a monovalent ion. Monovalent copper ion and the radical groups of tyrosine, …

Folin-酚法（Lowry法）最灵敏的 蛋白质测定 方法之一。 过去此法是应用最广泛的一种方法，由于其试剂乙的配制较为困难，近年来逐渐被 考马斯亮兰法 所取代。 最早由Lowry确定了 蛋白质 浓度测定的基本步骤。 以后在 生物化学 领域得到广泛的应用。 这种 蛋白质 测定法是最灵敏的方法之一。 过去此法是应用最广泛的一种方法，由于其试剂乙的配制较为困难（可订购），逐渐被考马斯亮蓝法所取代。 此法的显色原理与 双缩脲 方法是相同的，只是加入了第二种试剂，即Folin— …

Lowry法（Folin-酚试剂法）： 福林-酚试剂（Folin-phenol reagent）的显色原理是：首先在碱性条件下蛋白质与铜作用生成蛋白质-铜复合物，该复合物随之将磷钼酸- 磷钨酸 还原成蓝色复合物。

Lowry’s assay for total protein estimation is one of the most commonly used colorimetric assays. The Biochemist Oliver H. Lowry developed the reagent in the 1940s. His publication on the same in the year1951 was highly cited and has been used in protein labs. • To determine the concentration of proteins by Lowry’s method.

The cyt c concentration is most accurately measured using the spectral properties of the covalently bound heme prosthetic group in its reduced state. For other proteins, we use the Lowry protein assay method to determine protein concentration [22].

The most accurate method of determining protein concentration is probably acid hydrolysis followed by amino acid analysis. Most other methods are sensitive to the amino acid composition of the protein, and absolute concentrations cannot be obtained (1).

Lowry 法的显色原理是根据双缩脲反应，蛋白质中的肽键在碱性条件下与Cu2+反应，生成紫红色Cu+复合物。 Folin-酚试剂在Cu+的催化下，磷钼酸磷钨酸盐被蛋白质中的芳香族氨基酸残基还原，产生深蓝色的钼蓝和钨蓝混合物，其最大吸收峰在745~750nm处。

iversity of Florida The Lowry procedure is one of the most venerable and widely-used protein assays, being first described in 1951 [Lowry et al., J. Biol. Chem. 1. 3: 265-275 (1951)]. Under alkaline conditions, copper com. lexes with protein. When folin phenol reagent (phospho-molybdic-phosphotungstic reagent) is added, the Folin-phenol reagent b.

2013年10月13日 · Aim: To estimate the concentration of proteins by the Lowry method from the given sample. The blue color developed in the Lowry method results from the reduction of phosphomolybdic-phosphotungstic components in the Folin-Ciocalteau reagent. This reduction occurs due to the presence of the amino acids tyrosine and tryptophan in the protein.