MALDI-TOF mass spectrometry is a versatile analytical technique to detect and characterize mixtures of organic molecules. In microbiology, it is used as a rapid, accurate, and cost-effective method for identifying microorganisms (bacteria, fungi, and viruses).

2023年4月29日 · Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI – TOF MS) is an emerging potential tool used for the identification and diagnosis of microorganisms.

2023年8月8日 · Principle: MALDI-TOF MS is based on the combined principles of matrix-assisted desorption/ionization and time-of-flight analysis, enabling rapid and accurate determination of molecular masses. Sample Preparation: Samples are mixed with a matrix, which absorbs laser energy and facilitates ionization of the analyte molecules.

MALDI-TOF analysis is very fast • Can do TOF-TOF MS-MS analysis • BUT what happens chemically on the acidic environment on the surface of the plate during storage • Also, can the laser beam cause chemical changes?

MALDI-TOF To put the basic MALDI-TOF separation principle into simple words: The larger its m/z, the slower an ion will fly, the longer the measured flight time will be. This defines the potential energy at which all ions start from the MALDI target. This equation defines the kinetic energy of ions after acceleration into the flight tube.

2024年7月17日 · MALDI is the abbreviation for "Matrix Assisted Laser Desorption/Ionization." The sample for MALDI is uniformly mixed in a large quantity of matrix. The matrix absorbs the ultraviolet light (wavelength 337~355nm) and converts it to heat energy.

Mass spectrometry is an analytical technique in which samples are ionized into charged molecules and ratio of their mass-to-charge (m/z) can be measured. In MALDI-TOF mass spectrometry, the ion source is matrix-assisted laser desorption/ionization (MALDI), and the mass analyzer is time-of-flight (TOF) analyzer.

2016年8月19日 · This chapter reviews the history and the background of matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-ToF-MS) analysis. The chapter covers, application of this technique in variety of different areas with the special focus on...

Matrix-assisted laser desorption-ionization time of flight mass spectrometry (MALDI-TOF MS) is replacing traditional methods for identifying microorganisms in the clinical laboratory. This relatively simple technique overcomes many of the challenges of identifying bacteria and fungi.

MALDI is the abbreviation for "Matrix Assisted Laser Desorption/Ionization." The sample for MALDI is uniformly mixed in a large quantity of matrix. The matrix absorbs the ultraviolet light (nitrogen laser light, wavelength 337 nm) and converts it to heat energy.