McrBC is an endonuclease which cleaves DNA containing methylcytosine* on one or both strands. Sites on the DNA recognized by McrBC consist of two half-sites of the form (G/A)mC. …

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McrBC是一种作用于含有甲基胞嘧啶DNA的特异性核酸内切酶，不作用于非甲基化DNA。 它能够特异性地识别和切割包含两个 (G/A)mC间距在40 bp-2000 bp的DNA序列。 (G/A)mC中的甲基 …

2018年6月12日 · McrBC是一种检测基因组小部分区段的DNA甲基化情况的核酸内切酶。 只能降解甲基化的区域，对未甲基化区域不起作用。 酶切体系. 具体操作以及试剂信息相见 McrBC. 发 …

McrBC specifically cleaves DNA containing methyl cytosine (mC : 5-hydroxymethylcytosine, 5-methylcytosine, 4-methylcytosine) preceded by a purine nucleotide (Pu : A or G). DNA …

在50 μl 反应液中,37 °C下反应1 小时,将0.25 μg的含有单一McrBC 酶切位点的线型pBR322 (pBR322-mu-BamH I-M Alu I)完全分解,所需要的酶量定义为1 个活性单位(U)。 使用本产品作 …

DNA上McrBC的识别位点由两个 (G/A)mC形式的半位点组成，这两个半位点之间的距离可以达到3kb，但是最佳距离为55～103bp。 McrBC的切割需要GTP，当存在不可水解的GTP类似物 …

Mcr phenotypes reported in [Raleigh, E.A. et al. (1988) Nucl. Acids Res., 16, 1563-1575 PMID: 2831502, References 7 in Restriction of Foreign DNA by E. coli K-12] except those denoted …

I - Capa: apresenta numeração, data, base normativa e comando de atualização do MCR; II- Índice do MCR: relaciona os Capítulos, as Seções e a relação dos Documentos previstos no …

1992年5月20日 · McrBC-mediated restriction of modified DNA has been studied extensively by genetic methods, but little is known of its molecular action. We have used overproducing …