Peptide Synthesis - history, protecting groups, reagents, practical ...
2023年12月25日 · In solid-phase peptide synthesis (SPPS) one peptide end is attached to water-insoluble polymer and remains protected throughout the entire peptide formation, meaning both fewer steps and simplified purification, the reagents …
Solid-phase peptide synthesis The purpose of this step is to sequentially add amino acids to the resin to build a peptide chain. There are two main steps in coupling an amino to peptide chain. The first step is deprotecting Fmoc from the amino group on the amino acid on the resin to expose an amine. The second step is
Peptide Synthesis: Methods and Protocols | SpringerLink
Authoritative and practical, Peptide Synthesis: Methods and Protocols serves as an essential guide to the many crucial processes that will allow researchers to efficiently prepare, purify, characterize, and use peptides for chemical, biochemical, and biological studies.
2007年12月13日 · This protocol for solid-phase peptide synthesis (SPPS) is based on the widely used Fmoc/tBu strategy, activation of the carboxyl groups by aminium-derived coupling reagents and use of PEG-modified polystyrene resins.
Before preparing any peptide on a large scale, it should be synthesized on a small scale first to identify and rectify potential problems. Many books covering the theory and practice of solid phase synthesis have been published. The following are a few of the recent publications.
Guide to Solid Phase Peptide Synthesis - AAPPTEC
An introductory guide to solid phase peptide synthesis. Resins, amino acid derivatives, coupling reagents, common problems and their solutions, peptide cleavage from resins, peptide purification and storage are some of the topics discussed.
Planning a Peptide Synthesis - AAPPTEC
Before choosing a resin to use in a peptide synthesis, you must answer several questions first: 1) Will Boc-amino acids or Fmoc-amino acids be used to synthesize the peptide? 2) Will the product be a peptide acid or a peptide amide? 3) How large is the peptide?
Solid-phase peptide synthesis: from standard procedures to …
2007年12月13日 · This protocol for solid-phase peptide synthesis (SPPS) is based on the widely used Fmoc/tBu strategy, activation of the carboxyl groups by aminium-derived coupling reagents and use of...
12.8: Peptide Synthesis - Chemistry LibreTexts
2024年11月19日 · The strategy for peptide synthesis, as outlined here, should now be apparent. The following example shows a selective synthesis of the dipeptide Ala-Gly. An important issue remains to be addressed. Since the N-protective group is an amide, removal of this function might require conditions that would also cleave the just formed peptide bond ...
Peptide Synthesis - SpringerLink
2023年8月3日 · This article describes a generalized protocol for solid-phase peptide synthesis (SPPS) using Fmoc chemistry, including their purification and characterization by reversed-phase high-performance liquid chromatography and mass spectrometry.
- 某些结果已被删除