Using neutrophils from mice deficient in Phd3, we demonstrated a unique role for Phd3 in prolonging neutrophil survival during hypoxia, distinct from other hypoxia-associated changes in neutrophil function and metabolic activity.

phd3可增加m2型丙酮酸激酶(pkm2)和hif-1α的联系，激活多个结构域，包括hif-1α的反式激活结构域和pas结构域，pkm2激活hif的靶基因，在癌细胞中使得糖酵解增多 [24] 。所以，phd在hif依赖及非hif依赖的方式中可能是通过调节pkm2和pdh的活性来控制糖酵解的速率。

phd3是 脯氨酰羟化酶 家族的成员，可通过调节转录因子 hif1α 来调节与炎症和免疫控制中的许多信号通路，此外也发现phd3通过抑制长链脂肪酸进入线粒体来调节脂肪酸氧化（fao），结果表明肿瘤细胞和cd8+ tils对hfd全身性应激的代谢适应（包括脂肪代谢变化）有所 ...

We examined PHD expression in human neutrophils and found that PHD3 was strongly induced in response to hypoxia and inflammatory stimuli in vitro and in vivo. Using neutrophils from mice deficient in Phd3, we demonstrated a unique role for Phd3 in prolonging neutrophil survival during hypoxia, distinct from other hypoxia-associated changes in ...

2008年2月15日 · Oxygen-dependent hydroxylation of hypoxia-inducible factor (HIF)- α subunits by prolyl hydroxylase domain (PHD) proteins signals their polyubiquitination and proteasomal degradation, and plays a...

我们发现，PHD3表达在包括急性髓细胞性白血病（AML）在内的癌症子集中大大降低，并且与脂肪分解代谢的依赖性有关，而与外部营养因素无关。 PHD3的过表达通过调节ACC2限制了FAO，因此阻碍了白血病细胞的增殖。 因此， While much research has examined the use of glucose and glutamine by tumor cells, many cancers instead prefer to metabolize fats.

3 天之前 · A significant reduction in Sirt1 and Phd3 expression, along with an increase in Hif-1α and cleaved caspase-3, was observed in a time-dependent manner post-myocardial infarction (MI). In vitro findings revealed that hypoxia decreased nuclear Sirt1 and cytoplasmic Phd3 levels while promoting a time-dependent increase in Hif-1α and cleaved ...

The HIF prolyl hydroxylases (PHDs/EGLNs) are central regulators of the molecular responses to oxygen availability. One isoform, PHD3, is expressed in response to hypoxia and causes apoptosis in oxygenated conditions in neural cells. Here we show that PHD3 forms subcellular aggregates in an oxygen-dependent manner.

2020年8月4日 · PHD3-null mice demonstrate loss of ACC2 hydroxylation in heart and skeletal muscle and display elevated fatty acid oxidation. Whole body or skeletal muscle-specific PHD3 loss enhances exercise capacity during an endurance exercise challenge.

2020年12月13日 · 特别令人感兴趣的是phd3，一种在正常细胞中被证明可以抑制过度脂肪代谢的蛋白质。 与正常环境相比，肥胖环境下癌细胞的PHD3表达明显降低。 蛋白质水平分析证实高脂肪饮食肿瘤细胞脂肪摄入和氧化增强（来源：Cell）