Download and complete this form to establish a PRFA with a state or local government. Download and complete this form to amend an existing PRFA with either a federal or non-federal government agency. The sample Statement of Work (SOW) lists specific removal actions that are ordered through PRFAs.

2017年8月15日 · The Pollution Removal Funding Authorization (PRFA) is a tool available to FOSCs to quickly obtain needed services and assistance from other government agencies: federal, state, or local, as well as recognized Indian Tribes in oil spill and hazardous materials response actions.

2025年2月25日 · Hainzl et al. structurally explain the mechanism of promiscuous (sequence-independent) inhibition of Listeria virulence activator PrfA by environmental oligopeptides. Parallel and antiparallel β sheet-like interactions mediate nonspecific peptide docking at the binding site of the PrfA-activating cofactor, glutathione (GSH).

2011年4月1日 · PrfA, a regulatory protein, integrates a number of environmental cues that signal the transition between these two contrasting lifestyles, activating a set of key virulence factors during host infection. While a number of details concerning the general mode of action of this virulence master switch have been elucidated, others remain unsolved.

2024年12月1日 · Positive Regulatory Factor A (PrfA) is a well-studied transcription factor in L. monocytogenes that is responsible for activating a plethora of virulence factors. Targeting virulence factors is a promising strategy that is being considered to combat bacterial infections, hence targeting PrfA is both logical and attractive.

2020年12月8日 · PrfA functions as a master regulator of virulence in L. monocytogenes, controlling the expression of many virulence factors. The temperature-regulated expression of PrfA by its RNAT element serves as a signal of successful host invasion for the bacteria.

The PrfA thermoregulator UTR is an RNA thermometer found in the 5' UTR of the prfA gene. [1] In Listeria monocytogenes, virulence genes are maximally expressed at 37 °C (human body temperature) but are almost silent at 30 °C. [2] The genes are controlled by PrfA, a transcriptional activator whose expression is thermoregulated. [3]

Most virulence genes of Listeria monocytogenes are activated by the transcriptional regulator PrfA. Previous studies have shown that environmental parameters, such as temperature, pH, stress conditions and medium composition, affect the expression of …

PrfA, a key transcriptional activator that regulates most of the known listerial virulence gene expression, has been shown to promote L. monocytogenes biofilm formation. In this study, the whole-genome microarray was used to identify differentially expressed genes associated with the putative interaction between biofilm formation and PrfA in L ...

PrfA 是LM中迄今为止发现的唯一的一个调节 绝大多数毒力基因转录表达的蛋白. LM毒力岛中的prfA 基因编码, 分子量为27 kD。 很重要的作用。 α−螺旋组成的D 结构。 该结构在Crp 中介导与辅因. Fig. 1 The central gene cluster (LIPI-1) and other PrfA-regulated genes (modified from Kreft and Vazquez-Boland, 2001). P: promoter, an asterisk above means the presence of a PrfA-box within the promoter.