RPMI 1640, simply known as RPMI medium, is a cell culture medium commonly used to culture mammalian cells. [1] RPMI 1640 was developed by George E. Moore, Robert E. Gerner, and H. Addison Franklin in 1966 at Roswell Park Comprehensive Cancer Center (formerly known as Roswell Park Memorial Institute), from where it derives its name. [ 2 ]

Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications.

Choose RPMI 1640 Media in standard, innovative, or custom formulations to fit your cell culture needs. RPMI 1640 is vitamin-rich and contains the reducing agent glutathione.

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.

RPMI 1640 Medium is the only media that contains the reducing agent glutathione and high concentrations of vitamins and choline. It also contains vitamin B12, biotin, and PABA, which are not found in DMEM.

RPMI-1640 medium is commonly used for suspension cultures of mammalian and primary cells, such as HeLa cells, human peripheral blood mononuclear cells (PBMCs), human normal and neoplastic leukocytes, Jukrat cells, and more.

Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.

Roswell Park Memorial Institute (RPMI) medium or RPMI 1640 is used in cell culture, tissue culture, and also to improve the cellularity and morphology of CSF cytology specimens. To determine whether RPMI medium can be used to preserve cell morphology in pleural/peritoneal effusion samples.

Supports cell growth in research and biomanufacturing cell culture processes. HyClone RPMI 1640 medium is extensively quality control tested to ensure lot-to-lot consistency. Medium efficacy and homogeneity of RPMI 1640 is tested with HyClone sera. HyClone RPMI solution with L-glutamine is available with or without HEPES.

RPMI-1640 was developed by Moore, et al. at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins.