RNA-dependent RNA polymerase 6 is required for efficient hpRNA-induced ...
2021年2月3日 · RNA silencing induced by sense transgenes is dependent on RNA-dependent RNA polymerase 6 (RDR6), which converts single-stranded (ss) RNA into double-stranded (ds) RNA. By contrast, it has been proposed that RNA silencing induced by self-complementary hairpin RNA (hpRNA) does not require RDR6, because the hpRNA can directly fold back on itself ...
Plant Cell | 中科院遗传发育所程祝宽研究组发现RDR6调控减数分 …
rdr6是重要的rna依赖rna聚合酶，在小rna的生物发生过程中发挥着重要的功能。 水稻rdr6突变体是一经典的致死突变体，其强等位突变体由于影响胚芽的发育而不能萌发成苗，因而对其在整个生命周期中的功能尚缺乏足够认识。
Biochemical Activities of Arabidopsis RNA-dependent RNA Polymerase 6
In Arabidopsis, genetic evidence demonstrates that RNA-dependent RNA polymerase 6 (RDR6) plays a fundamental role in at least four RNA silencing pathways whose functions range from defense against transgenes or viruses to endogene regulation in development and in …
The cellular RNA-dependent RNA polymerases in plants
2024年8月13日 · RDR6 plays an essential role in PTGS maintenance. In addition, it participates in antiviral responses, transgene silencing, and various developmental processes (Willmann et al., 2011). RDR6 mainly converts Pol II-produced ssRNA into dsRNA.
Analysis of RDR1/RDR2/RDR6‐independent small RNAs in …
2018年4月14日 · Known RDR1 / 2 / 6 -dependent sRNAs include phased and repeat-associated short interfering RNAs, while known RDR1 / 2 / 6 -independent sRNAs are primarily microRNAs (miRNA) and other hairpin-derived sRNAs. In this study we produced and analyzed sRNA-seq libraries from rdr1 / rdr2 / rdr6 triple mutant plants.
分子植物卓越中心揭示活跃转座子识别及表观沉默机制----中国科 …
2021年3月2日 · 而激活的或未被甲基化修饰的转座子则通过RNA依赖的RNA聚合酶6（RDR6）介导产生21-22 nt的siRNA，触发RNAi和从头开始的DNA甲基化，这一过程称为RDR6-RdDM。 RDR6-RdDM对于引发活跃转座子的沉默十分重要，但RDR6如何特异识别转座子RNA并选择性加工siRNA未知。 此前研究表明，mRNA的初始切割是RDR6识别的关键先决条件，且siRNA的产生在细胞空间上局限于RDR6和SGS3共定位的siRNA小体中。 研究表明，植物转座子RNA由于 …
The poly (A) tail blocks RDR6 from converting self mRNAs into ... - Nature
2017年3月20日 · Here, we show that RDR6 itself selects aberrant poly (A)-less mRNAs over canonical polyadenylated mRNAs as templates at the initiation step of complementary strand synthesis. This mechanism can...
Involvement of RDR6 in short-range intercellular RNA silencing …
2012年6月22日 · Using a local RNA silencing assay, we report on a distinct mechanism that may govern the short-range (6–10 cell) trafficking of virus-induced RNA silencing from epidermal to neighbouring palisade...
RNA-directed DNA methylation: an epigenetic pathway of
2014年5月8日 · Recent research has uncovered variations on the canonical RdDM pathway, including the involvement of RNA-DEPENDENT RNA POLYMERASE 6 (RDR6) and NEEDED FOR RDR2-INDEPENDENT DNA METHYLATION (NERD),...
Rice RNA-dependent RNA polymerase 6 acts in small RNA ... - PubMed
Higher plants have evolved multiple RNA-dependent RNA polymerases (RDRs), which work with Dicer-like (DCL) proteins to produce different classes of small RNAs with specialized molecular functions. Here we report that OsRDR6, the rice (Oryza sativa L.) homolog of Arabidopsis RDR6, acts in the biogene …