2024年4月10日 · Here, we present the cryo–electron microscopy structures of the budding yeast Rpd3S complex in both its apo and three nucleosome-bound states at atomic resolutions, revealing the exquisite architecture of Rpd3S to well accommodate a …

2000年1月6日 · Rpd3 negatively regulates meiosis and is required for repression of certain meiotic genes during vegetative growth. In diploid cells, mutations in RPD3 cause defects in mitotic recombination and in sporulation. Two distinct Rpd3 complexes have been identified: a small Rpd3S complex and a large Rpd3L complex.

2023年7月19日 · Here we solved the cryo-electron microscopy structures of Saccharomyces cerevisiae Rpd3S in its free and H3K36me3 nucleosome-bound states. We demonstrated a unique architecture of Rpd3S, in which...

The RPD3 family is the most widely studied HDACs. This article summarizes the regulatory mechanisms of Arabidopsis RPD3 family in several growth and development processes, which provide a reference for studying the mechanisms of RPD3 family members in …

2023年9月4日 · In Saccharomyces cerevisiae, cryptic transcription at the coding region is prevented by the activity of Sin3 histone deacetylase (HDAC) complex Rpd3S, which is carried by the transcribing RNA...

Sin3作为支架蛋白和 Rco1亚基一起协调了复合物的组装，并通过其 HID、 MD和 CTD结构域包裹住催化亚基 Rpd3；同时， Sin3-DNA、 Rco1-linker DNA结合界面共同帮助引导 Rpd3S精准锚定在核小体底物上。

2023年5月27日 · Here we present the structure of the S. cerevisiae Rpd3L complex determined by cryogenic electron microscopy (cryo-EM). The complex contains an asymmetric dimeric core that can be divided into two...

2021年12月21日 · RPD3 is a well-conserved class I histone deacetylase (HDAC) that is involved in diverse biological processes. Here, we investigated the roles of the Magnaporthe oryzae RPD3 (MoRPD3) gene, an ortholog of Saccharomyces cerevisiae Rpd3, during development and pathogenesis in the model plant-pathogenic fungus Magnaporthe oryzae.

2012年1月10日 · We investigated the HDAC-independent properties of the Rpd3 complexes biochemically and discovered a chaperone function, which promotes histone deposition onto DNA, and a novel activity, which prevents nucleosome eviction but not remodeling mediated by the ATP-dependent RSC complex.

2024年8月1日 · 在酿酒酵母中，组蛋白去乙酰化酶Rpd3作为抑制因子调控基因转录，参与形成Rpd3S（Small）和Rpd3L（Large）两种分子量约为0.6MDa和1.2MDa的大分子复合物来执行抑制基因转录的功能。