2024年10月8日 · Dynamic light scattering (DLS) and negative stain electron microscopy (nsEM) revealed that all eight monovalent RBD-NPs (four variants with and without Rpk9 mutations) were of the expected...

RPK-9 Medvedka ("Mole cricket", NATO Designation SS-N-29) [1][2] is a modern missile system used to engage submarines. The system consists of a launcher with eight missiles, each with …

Scaled-up expression from HEK293F cells and purification by immobilized metal affinity chromatography (IMAC) and size exclusion chromatography (SEC) confirmed increased yields for Rpk4 and Rpk9 both as monomers and as fusions to the I53-50A trimer, with Rpk9 showing a clear advantage for I53-50A trimers (Figure 2A and Supplementary Figure 2A ...

2022年11月10日 · SARS-CoV-2 S 1 has a mu-phosphatase signal peptide, mutated furin cleavage site to SGAS, D614G mutation, and Rpk9 mutations (Y365F/F392W/V395I) and ends at S686, followed by a 16GS linker, I53-50A, and C-terminal his tag.

2024年10月8日 · Pink spheres indicate β and γ RBD mutations (K417N/T, E484K, and N501Y). Purple spheres indicate stabilizing Rpk9 mutations (Y365F, F392W, and V395I). Representative DLS of 8 monovalent RBD-NPs with and without Rpk9 mutations.

Notably, the “rpk9” combination of stabilizing mutations (Y365F, F392W, V395I) described for the Wuhan-Hu-1 RBD also enhanced yeast-surface expression of the Omicron BA.1 RBD . Epistatic shifts in the Omicron mutational landscape

2021年6月1日 · Nanoparticle immunogens presenting the receptor-binding domain (RBD) of the SARS-CoV-2 Spike protein (S) in repetitive arrays are being advanced as second-generation vaccine candidates, as they...

2014年10月24日 · 为了向苏联海军的舰艇提供现代化的反潜 武器，它不占用太多空间，于八十年代初期启动了RPK-9项目。 新系统的开发委托给莫斯科热工学院（MIT）。 N. Mazurov成为项目经理。 最初，新项目名为Metelitsa，但为避免与URPK-3和URPK-4 Metel系统混淆，该项目很快改名为Medvedka。 在开发新项目时，决定采用一些旨在减少反潜艇综合体的尺寸和重量的新思路。 结果，麻省理工学院的设计人员设法创建了一种可以在排水量为350吨的船舶上使用的系统。 国 …

Combining targeted proteomics and metabolomics suggests that acquisition of resistance in RPK9 cells is due to attenuation of dFdC phosphorylation via suppression of dCK.

2011年11月7日 · The RPK-9 Medvedka [SS-N-29] is similar to the American ASROC-like torpedo carrying rocket for small ships. The MEDVEDKA Missile System is intended to engage hostile submarines and can be...