RPN9 1 Systematic Name YDR427W SGD ID SGD:S000002835 Aliases NAS7 6 Feature Type ORF , Verified Description Non-ATPase regulatory subunit of the 26S proteasome; similar to putative proteasomal subunits in other species; null mutant is temperature ...

蓝色表示rpn9 的wh 亚结构域，紫色、绿色和橙色分布表示rpn6（pdb id：3txn）、 rpn12（pdb id：4b0z）和csn7（pdb id：3chm）的wh 亚结构域。

本项目拟通过解析lid中两个含PCI结构域的蛋白Rpn5和Rpn9的二元复合物晶体结构，并基于结构分析，运用生物化学和生物物理学等手段来阐明含PCI 结构域蛋白之间的相互作用方式，为进一步阐明lid等复合物的组装机制奠定结构基础。

RPN9 / YDR427W Protein Protein abundance data, domains, shared domains with other proteins, protein sequence retrieval for various strains, sequence-based physico-chemical properties, protein modification sites, and external identifiers for the protein.

2022年12月15日 · RPN9 is a full-featured RPN (reverse Polish notation) scientific calculator. It is designed for engineers, scientists, college students and advanced high-school students.

2025年2月8日 · proteasome regulatory particle lid subunit RPN9. GeneRIFs: Gene References Into Functions. Rpn10, but not ubiquitylated-Rpn10, binds Rpn9 in vitro: ubiquitylation functions to dissociate modified ubiquitin receptors from their targets, a function that promotes cyclic activity of ubiquitin receptors

2022年10月17日 · Rpn9 C-terminal helix The 26S proteasome is the major ATP-dependent protease in eukaryotes which plays a key role in intracellular protein degradation. The lid of the 26S proteasome contains six PCI-domain-containing proteins (Rpn3/5/6/7/9/12), two MPN-domain-containing proteins (Rpn8/11), and one peptide, Sem1.

Rpn9 is a PCI domain-containing lid subunit and is necessary for the integrity and efficiency of the 26 S proteasome (29, 30). It interacts with the ubiquitin receptor Rpn10, and the Δrpn9 Saccharomyces cerevisiae strain was reported to accumulate multiubiquitinated proteins at restrictive temperatures .

Conclusion: The Rpn9-Rpn5 interaction is contributed by a hydrophobic center and surrounding ionic pairs in their PCI domains. Significance: The results provide structural insights into lid assembly regulation via PCI-PCI interactions.

RPN9 encodes a subunit of the 26S proteasome, a highly conserved eukaryotic protease responsible for intracellular protein degradation. The 26S proteasome consists of a 20S catalytic core particle (CP) and a 19S regulatory particle (RP), and controls many fundamental biochemical processes by programmed degradation of regulatory protein targets ...