RNA splicing is a process in molecular biology where a newly-made precursor messenger RNA (pre- mRNA) transcript is transformed into a mature messenger RNA (mRNA). It works by removing all the introns (non-coding regions of RNA) and …

2023年6月20日 · The GU is the 5' splice site (sometimes called the donor splice site) and the AG is the 3' splice site (or acceptor splice site). GU is invariant at the 5' splice site, and AG is (almost) invariant at the 3' splice site for the most prevalent class of introns in pre-mRNA.

Introns are removed from primary transcripts by cleavage at conserved sequences called splice sites. These sites are found at the 5′ and 3′ ends of introns. Most commonly, the RNA sequence...

In general, mutations in the canonical acceptor and donor sites affect strongly conserved sequences that define exon-intron boundaries. The 5′ splice site (CAG/GUAAGU sequence) and 3′ splice site (NYAG/G sequence) are recognized by the elements of the spliceosome.

2025年1月15日 · The established consensus sequence for human 5′ splice sites masks the presence of two major splice site classes defined by preferential base-pairing potentials with either U5 snRNA loop 1 or the U6 snRNA ACAGA box.

Posttranscriptional modifications on newly synthesized mRNA are imperative for the translation into a functional protein. mRNA splicing is an integral part of pre-mRNA processing that involves removing non-coding stretches and merging the coding sequences before transport to …

Splice site consensus sequences vary by species. Consensus sequences for humans and flies are reported here. A survey (making the point that species with more introns have weaker splice sites) was published by Irimia et al. . Splice site predictors are available on the web. I …

Splice site selection is fundamental to pre-mRNA splicing and the expansion of genomic coding potential. 5′ Splice sites (5′ss) are the critical elements at the 5′ end of introns and are extremely diverse, as thousands of different sequences act as bona fide 5′ss in the human transcriptome.

ACCEPTOR-SPLICE: splicing site at the end of an intron, intron 3' right end. The GT/AG mRNA processing rule is applicable for almost all eukaryotic genes [1,2]. A polypyrimidine (C n T n) motif is present upstream of the CAG intron 3' ending.

Search for known mammalian splice site sequences and other related information. Search for occurrences of the four major alternative-splicing modes in human genome. A web-based tool for splice-site analysis. Search for information on alternative splice events at GYNGYN donors and NAGNAG acceptors.