V (D)J recombination (variable–diversity–joining rearrangement) is the mechanism of somatic recombination that occurs only in developing lymphocytes during the early stages of T and B cell maturation. It results in the highly diverse repertoire of antibodies/immunoglobulins and T cell receptors (TCRs) found in B cells and T cells, respectively.

2018年11月29日 · We present the largest-to-date VDJH gene repertoire analysis in MM, consisting in biological and clinical data from 413 patients.

2001年11月1日 · In the present study, we report on the gene scanning technique for size fragment analysis of VDJH-rearranged products in order to detect clonally rearranged cells in the apheresis products of MM...

The gene scanning of clonally rearranged VDJ segments of the heavy chain immunoglobulin gene (VDJH) is a reproducible and easy to perform technique that can be optimised for clinical laboratories.

2018年11月29日 · VDJH usage somatic hypermutation levels and CDR3 composition in multiple myeloma resembles the normal mature B cell repertoire. In contrast to Chronic Lymphocytic Leukemia (CLL), Mantle Cell Lymphoma or Marginal Zone Lymphoma, stereotyped receptors were absent in MM, which shows no evidence of autoantigen-driven selection.

2020年2月6日 · Here, we present the largest-to-date analysis of the VDJH repertoire in multiple myeloma, consisting in biological and clinical data from 413 patients diagnosed and treated according to the...

2000年8月4日 · In the present paper, we report on the use of the heteroduplex PCR technique to detect the presence of clonally rearranged VDJ segments of the heavy chain immunoglobulin gene (VDJH) in the...

In the present paper, we report on the use of the heteroduplex PCR technique to detect the presence of clonally rearranged VDJ segments of the heavy chain immunoglobulin gene (VDJH) in the apheresis products of patients with multiple myeloma (MM) undergoing autologous peripheral blood stem cell (APB …

互补决定区是VL与VH均有3个HVR，它们共同组成Ig的抗原结合部位（antigen-binding site）。 该部位因在空间结构上可与抗原决定簇形成精密的互补，故超变区又称互补性决定区 (complementarity determining region，CDR)。 整个抗体分子可分为恒定区和可变区两部分。 在可变区内有一小部分氨基酸残基变化特别强烈，这些氨基酸的残基组成和排列顺序更易发生变异区域称高变区。 在L链、H链的V区中有三个高变区 (hypervariable regions，HVR)，该部位因在空 …

1989年9月8日 · We have constructed a hybrid immunoglobulin (VDJH)-T cell receptor (C alpha) gene using the VDJH exon from a digoxin-specific antibody. This gene was used to make a line of transgenic mice. The hybrid VDJH-C alpha protein is expressed on a subset of T cells in these mice, and we have shown that it f …