The composition and application of a single, chemically defined medium or growth and sporulation of Bacillus subtilis is described. At 37 degrees C cells grew with a doubling time of about 40 min; cultures attained near-maximal spore formation (70 ...

2022年5月20日 · Difco sporulation media was identified as the preferred sporulation medium for 4 out of 7 tested Bacillus strains. Sporulation rates in B. cereus, B. sphaericus, and B. thuringiensis were higher at 30°C than the rates at 37°C at a difference of 5%, 65%, and 20%, respectively.

In forward and side scatter you can easily distinct the difference between the purified and unpurified samples (figure 5). Purification leads to a better and solid read out in further experiments and applications. Figure 7. Bacillus subtilis spore purification. (A) The spores of Bacillus subtilis WT168 were analyzed using flow cytometry.

2022年6月6日 · Bacillus subtilis spores have important biological applications; however, high spore-cell densities and sporulation efficiencies in fermentation is poorly reported. This study systematically analyzed the spore densities and formation efficiency of B. subtilis BSNK-5 in different culture substrates.

Bacillus subtilis is also able to form endospores under distress (see sporulation protocol), which is one of the most efficient adaptations to lack of nutrients. These endospores are a highly stable form of the bacterium which are resistant to heat, UV-light and pressure. Under normal conditions they are able to re-enter their normal life

The composition and application of a single, chemically defined medium or growth and sporulation of Bacillus subtilis is described.

2013年8月8日 · In a fed-batch fermentation set-up, in which sporulation of ultraviolet (UV)-mutagenized B. subtilis is repeatedly triggered by periods of starvation, fitter strains with mutated tagE evolved....

2014年1月1日 · The deletion of msmX, which is involved in arabinose transport in the Bacillus genus, decreased growth and sporulation by 53.71% and 86.46% compared with wild-type strain NCD-2, respectively.

2005年11月14日 · Bacillus cereus ATCC 14579 endospores were produced in Y1 medium, a nutrient-rich, chemically defined sporulation medium, and in modified G medium, containing low amounts of nutrients. The average transcription level of the seven ger operons per cell was 3.5 times higher in Y1 medium, and the spores grown in this medium showed an enhanced ...

Here we report that a gastrointestinal isolate of B. subtilis sporulates with high efficiency during growth, bypassing the cell density, nutritional, and other signals that normally make sporulation a post-exponential-phase response.