A general pHLA-CD80 scaffold fusion protein to promote efficient ...
2024年9月19日 · Contrarily, the promotion of more evenly distributed CD4 + and CD8 + T cell proliferation was aided by the presentation of a single activation signal (pHLA scaffold and …
基于pHLA的免疫治疗平台直接调节抗原特异性T细胞|免疫治疗|细 …
2023年6月25日 · 然而，疫苗的效力取决于抗原提呈细胞 (APC)的功能，包括运输、抗原处理、共刺激与共抑制以及对肿瘤免疫抑制的敏感性。 例如，DC是一种APC，通过递送pHLA和强大 …
Expanded Antigen-Specific Elimination Assay to Measure Human CD8
2024年7月18日 · Resuspend cells in FACS buffer. Optionally, before surface staining, add 2-4 nM APC-conjugated peptide-HLA (pHLA) multimer (s) of interest to the effector-only and/or CFSE …
TCR mimic compounds for pHLA targeting with high potency …
The TCRs expressed by CD8 killer cells recognize pHLA complexes on tumor cells and are necessary and sufficient to mediate durable remissions across various types of cancer …
CD8-dependent cooperation of noncognate and cognate pHLA …
Similar experiments with mutated cognate pHLA-A2 resulted in almost linear dependence (Fig. 4 g ), suggesting that the effect of enhancement is mostly determined by CD8–pMHC-I interactions.
Structural basis of the TCR-pHLA complex provides insights into …
2023年2月1日 · In this study, we investigated the mechanism of the high-affinity CD8 + T cell response to immunodominant HIV-1 epitope Gag-TL9 by reporting its TCR-pHLA ternary …
CD8 T cell function and cross-reactivity explored by stepwise …
2022年8月10日 · Finally, we took advantage of a collection of T cells expressing affinity-optimized NY-ESO-1-specific TCRs to interrogate the individual and combined impact of peptide:HLA …
Binding of noncognate QD ͞ pHLA-A2 conjugates to live CTL is CD8 ...
The mutation in cognate pHLA-A2 significantly decreased the binding of QDGL9- HLA-A2 conjugate to live CTL as well. These experiments clearly show a significant contribution of …
Development of a CD8 co-receptor independent T-cell receptor specific ...
CD8 molecules are known to significantly increase the TCR-based recognition of peptides presented by HLA class I molecules. 20 This CD8 dependency, however, can be overcome by …
Computational design and crystal structure of an enhanced …
2007年4月1日 · Human CD8 is a T cell coreceptor, which binds to pHLA I and plays a pivotal role in the activation of cytotoxic T lymphocytes. Soluble recombinant CD8 alphaalpha has been …