Plasmid pCL-Eco from Dr. Inder Verma's lab contains the insert gag/pol/env and is published in J Virol. 1996 Aug . 70(8):5701-5. This plasmid is available through Addgene.

逆病毒包装载体pCL-Eco是单质粒包装系统，快速产生Helper-free、毒力高的逆病毒颗粒。 450 ml DMEM (JRH Biosciences) 50 ml FBS (JRH Sciences) 5 ml Glutamine (200 mM in saline, JRH Sciences) 2.5 ml Gentamycin (10 mg/ml in water, Sigma) HC11 Growth Medium: 5 ml L-Glutamine (200 mM in saline, JRH Sciences) 2.5 ml Bovine Insulin (1 mg/ml in …

2021年3月16日 · 逆病毒包装质粒 ，pCL-Eco/pCL-10A1/pCL-Ampho是单质粒包装系统，快速产生Helper-free、毒力高的逆病毒颗粒。 pCL-Eco(适合鼠源不适合人源细胞) pCL-Eco

自认为如果感染大小鼠细胞的话，pCL-Eco时辅助质粒首选。 如果用VSVG的话，敢接专门的pRetro-GagPol搭配pMD2.G效果最好。 另外，最近有试过Clontech的那套系统，GP2-293细胞还是不错的，相当于稳转了Gag.Pol的HEK293T。

The pCL-ECO packaging vector is a part of the RetroMax expression system and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. By introducing a retroviral vector into a cell expressing retroviral proteins, retroviral particles (virions) are shed into the culture medium at the ...

2024年11月15日 · 病毒的包膜蛋白决定了病毒侵染的宿主范围，目前，根据逆转录病毒基因转移系统的亲嗜性，可分为4种：① 单嗜性 (Ecotropic)，包膜蛋白为env (gp70)，只能感染小鼠和少数品种的大鼠细胞；② 双嗜性 (Dualtropic)，包膜蛋白采用鼠白血病病毒的包膜蛋白4070A，能同时感染小鼠和人的细胞；③ 多嗜性 (Amphotropic)，包膜蛋白采用鼠白血病病毒的包膜蛋白10A1，既能感染小鼠和人的细胞，也能感染其它哺乳动物细胞；④ 泛嗜性 (Pantropic)，包膜蛋白采用水 …

Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence). Addgene has sequenced portions of this plasmid for verification. The results are shown below. Leading primers are indicated on the first line of each sequence.

The pCL-ECO packaging vector is a part of the RetroMax expression system and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system.

提供 pCL-Eco 载体/质粒图谱，全长序列，抗性，大小和元件信息。

pCL-Eco质粒 货号：kl-zl-0981 规格：20ul. The pCL-ECO packaging vector is a part of the RetroMax expression system and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system.