非对角线表明两个氢存在耦合。7:HSQCHeteronuclear Multiple-Quantum Correlation (HMQC）,HMQC是H检测的直接的异核化学位移相关谱。F维代表IH的化学位移，F1维代表13C的化学位移，所给出的信息与直接检测的C H-COSY谱基本相同。在实验时可采用13C去偶和不去偶 …

2012年10月24日 · All H-C correlations are shown. It can be useful as a basis for picking a 3D 13 C NOESY spectrum. It can also be a useful reference for the aromatic peaks.

(1 H, 13 C)-HSQC or HMQC serves to identify proton-bearing carbons and to associate these carbons with their attached protons. HSQC spectra feature generally better line shapes than HMQC and the commonly used multiplicity-edited HSQC versions offer the added benefit of distinguishing CH 3, CH 2, and CH groups.

2023年2月11日 · In an HSQC spectrum, a 13 C or heteroatom spectrum is displayed on one axis and a 1 H spectrum is displayed on the other axis. Cross-peaks show which proton is attached to which carbon or heteroatom. The purpose of a HSQC is to determine which protons are coupled to what other specific carbon or heteroatom in the molecule through bonds.

Gained Information: The Heteronuclear Single Quantum Correlation (HSQC) NMR experiment is a proton-detected 2D experiment that shows you 1H / 13C one-bond connectivity. You will see your 1H spectrum as your direct F2 axis and your 13C spectrum as your indirect F1 axis.

The heteronuclear single quantum coherence or heteronuclear single quantum correlation experiment, normally abbreviated as HSQC, is used frequently in NMR spectroscopy of organic molecules and is of particular significance in the field of protein NMR. The experiment was first described by Geoffrey Bodenhausen and D. J. Ruben in 1980. [1] .

1．H-CCOSY谱是相关1 H和13 CNMR信号的常规图谱，图谱比较直观，容易分析。 其作用与HMQC和HSQC相同。 2．HMQC（检测氢的异核多量子相干谱，1H-detected heteronuclear multiple-quantum coherence）和HSQC（检测氢的异核单量子相干谱，1H-heteronuclear single-quantum coherence）,这两种图谱的作用与H-CCOSY相当， 由于是反向实验，灵敏度高。 二者相比HSQC优点更多一些。 当样品量较多时，宜做H-CCOSY. 1．H-CCOSY谱是相关1 H和13 …

The differences between the two experiments are: (1) HSQC sequence involves more pulses, potentially making pulse calibration more important, and (2) more significantly, the HMQC spectrum also shows through-bond 1H-1H couplings along the indirect (13C) dimension because both 1H and 13C are in the XY plane during 13C evolution.

2D Gradient Enhanced 1H-13C HSQC. The following figure is the Varian gradient selected 2D 1H-13C HSQC pulse sequence (gHSQC) with the main parameters displayed (NMR500). Temperature must be regulated. Turn spin OFF. Gradients amplifier should be turned on (displays RUN). Type pfgon='nny' su to allow gradient pulses.

2015年6月5日 · 2D HSQC NMR is a very sensitive technique in liquid state that correlates chemical shifts of hetero bound nuclei. In 1 H– 13 C HSQC the magnetization from 13 C is transferred to 1 H, improving the sensibility of 13 C (McKenzie, Charlton, …