2014年3月3日 · Eukaryotic 18S ribosomal RNA (rRNA) gene primers that feature a wide coverage are critical in detecting the composition of eukaryotic microscopic organisms in ecosystems. Here, we predicted 18S rRNA primers based on consecutive conserved sites and evaluated their coverage efficiency and scope of application to different eukaryotic groups.

The primers target the 18S SSU rRNA and are based on those of Amaral-Zettler et al. (2009) and Stoek et al. (2010). The constructs are designed to be used with the Illumina platform. The forward primer, 1391f, is a universal primer, while the EukBr reverse primer favors eukaryotes, but can, with mismatch(s), bind and amplify bacteria and archaea.

The overall aim of the study was to find the best possible set of universal eukaryote 18S rRNA primers, which could be used as standard primers in biodiversity studies. To achieve this goal, >50,000 eukaryotic sequences from the SILVA database were used for …

The QuantumRNA™ 18S Internal Standards contain 18S rRNA primers and competimers designed to amplify 18S rRNA in all eukaryotes. The Universal 18S Internal Standards function across the broadest range of organisms including plants, animals and many protozoa.

2018年11月20日 · Several fungi-specific primers target the 18S rRNA gene sequence, one of the prominent markers for fungal classification. The design of most primers goes back to the last decades. Since then, the number of sequences in public databases increased leading to the discovery of new fungal groups and changes in fungal taxonomy.

Mouse Rn18s qPCR Primer Pair，即小鼠Rn18s qPCR引物对，主要用于基于SYBR Green的qPCR、One-Step qRT-PCR或semi-quantitative PCR。 本引物为预先设计、经过qPCR验证、预混的引物对。 qPCR (Quantitative PCR)即定量PCR，也称实时荧光定量PCR或实时定量PCR (Real-time quantitative PCR)、实时PCR (Real-time PCR)，是一种在DNA扩增反应过程中，以荧光定量测定每个聚合酶链式反应 (PCR)循环后产物总量的方法。 qPCR常用的两种方法 …

Although high-throughput amplicon sequencing is a powerful tool for the taxonomic profiling of soil nematodes, polymerase chain reaction (PCR) primers for amplification of the 18S ribosomal RNA (SSU) gene and preparation of template DNAs have not been sufficiently evaluated.

2014年3月3日 · Here, we predicted 18S rRNA primers based on consecutive conserved sites and evaluated their coverage efficiency and scope of application to different eukaryotic groups. After evaluation, eight...

2019年10月31日 · We found that widely-used 18S rDNA primers can amplify numerous stretches of the bacterial 16S rRNA gene, preventing the high-throughput detection of rare eukaryotic species, particularly in...

We built a database listing 285 primers and 83 unique primer pairs that have been used for eukaryotic 18S rRNA gene metabarcoding. In silico performance of primer pairs was tested against two sequence databases: PR 2 version 4.12.0 for eukaryotes and a subset of silva version 132 for bacteria and archaea.