2001年8月10日 · DNA methylation is one of the best-studied epigenetic modifications of DNA in all unicellular and multicellular organisms. In mammals and other vertebrates, methylation occurs predominantly at the symmetrical dinucleotide CpG (1-4).

2022年5月20日 · hek-293人胚肾细胞 ：是用 5 型腺病毒 75 株系转化，含有 ad5 e1 区的人胚肾亚三倍体细胞系，是一种 e1 区缺陷互补细胞系。 它是加拿大 McMaster University 的 F.L.Graham 与 J.S.Miley 于 1976 年用 DNA 转染技术构建而成。

Science 293 (5532), 1089-1093, 2001. 3911: 2001: Genomic imprinting: parental influence on the genome. W Reik, J Walter. Nature Reviews Genetics 2 (1), 21-32, 2001. 2883: 2001: Stability and flexibility of epigenetic gene regulation in mammalian …

2001年8月10日 · DNA methylation is one of the best-studied epigenetic modifications of DNA in all unicellular and multicellular organisms. In mammals and other vertebrates, methylation occurs predominantly at the symmetrical dinucleotide CpG (1–4).

2018年5月1日 · In this review, we provide an overview of the enormous morphological, taxonomical and functional diversity of soil protists, and discuss current challenges and opportunities in soil protistology. Research in soil biology would clearly benefit from incorporating more protistology alongside the study of bacteria, fungi and animals.

2022年10月30日 · Conclusions: In patients with TRH and chronic kidney disease compared with spironolactone with or without patiromer, finerenone was associated with a lower SBP reduction and lower risk of hyperkalemia and treatment discontinuation. Trial Registration: AMBER (NCT03071263), FIDELIO-DKD (NCT02540993), FIGARO-DKD (NCT02545049).

2001年8月10日 · Epigenetic modification of the genome ensures proper gene activation during development and involves (i) genomic methylation changes, (ii) the assembly of histones and histone variants into nucleosomes, and (iii) remodeling of other chromatin-associated proteins such as linker histones, polycomb group, nuclear scaffold proteins, and transcriptio...

2024年12月20日 · 293细胞转染是一种常用的细胞生物学技术，用于将外源DNA或RNA导入293细胞。 这种方法广泛应用于研究基因表达、蛋白质功能以及药物筛选等领域。 中科百抗PolyToolTM 293转染试剂适合于HEK293细胞及其他HEK293细胞的高密度悬浮培养、DNA瞬时转染及蛋白表达。 （一）使用PolyToolTM 293转染293细胞流程. 1.细胞接种（以25 mL体系为例） 根据细胞状态，选择合适的接种密度，建议 接种密度为1×106 cells/mL，待第二天细胞密度达到2×106 …

2002年1月15日 · The 293 cell line and two genetic variants, either expressing the SV40 large T-antigen (293T) or the Epstein–Barr virus (EBV) EBNA1 protein (293E), were tested for protein expression. The highest expression level was obtained with 293E cells using the EBV oriP-containing plasmid pCEP4.

The CT 293 Cyclotec™ laboratory mill is ideal for rapid and flexible preparation of a wide variety of feeds, grains, leaves and more for NIR or reference analysis. Excellent for all types of sample preparation when fineness and uniformity of particle size is essential.