293e细胞指的是在293-h细胞中插入ebna-1基因的细胞系，该基因能够稳定表达ebv病毒的ebna1蛋白。含有ebv复制起点（orip）的重组表达质粒，能够在h293e细胞中复制，并实现蛋白的高效表达。 293-6e细胞

Knockout achieved by using CRISPR/Cas9, Homozygous: 127 bp deletion in exon 2.

HEK-293人胚肾细胞 ：是用 5 型腺病毒 75 株系转化，含有 Ad5 E1 区的人胚肾亚三倍体细胞系，是一种 E1 区缺陷互补细胞系。它是加拿大 McMaster University 的 F.L.Graham 与 J.S.Miley 于 1976 年用 DNA 转染技术构建而成。 该细胞为人类腺病毒载体扩增的宿主。

2017年4月18日 · Our study showed that SYK, but not breast tumor kinase (BRK) or Bruton’s tyrosine kinase (BTK) was able to specifically phosphorylate MyD88 (Figure 6A). We next examined the tyrosine residues on MyD88 that were phosphorylated by SYK by co-transfecting WT or mutant MyD88 along with SYK in HEK293 cells.

2025年1月1日 · Spleen tyrosine kinase (SYK) exhibits diverse biological activities and participates in complex signaling pathways. SYK has vital roles in cancer, autoimmune diseases and other disorders. An overview of the development of SYK inhibitors hints at routes for future drug discovery efforts.

293T细胞最初称为293tsA1609neo，是293 [HEK-293]细胞株插入了SV40 T-antigen的温度敏感基因形成的高转衍生株。 hek-293t细胞能够复制携带SV40复制区的载体，用于生产逆转录病毒时，会产生高滴度病毒。

Firstly, we demonstrated the activation of the IRG1-itaconate axis induced by hvKP, with a dependency on SYK signaling rather than STING. Importantly, we discovered that exogenous supplementation of itaconate effectively inhibited excessive inflammation by directly inhibiting SYK kinase at the 593 site through alkylation.

2024年12月7日 · 脾酪氨酸激酶 （SYK） 是一种重要的非受体酪氨酸激酶，参与调节各种细胞过程的信号通路。 它主要在造血细胞和骨髓细胞中表达，对 B 细胞发育、成熟和抗体产生至关重要，是自身免疫和过敏性疾病的关键治疗靶点。 SYK 的过表达还与癌症和心血管、脑血管和神经退行性疾病有关，有助于它们的发生和发展。 SYK 是一个很有前途的药物开发靶点，并且已经报道了几种抑制剂。 本综述涵盖了 SYK 的结构和调节途径，以及它与各种疾病的联系。 它还重点 …

2018年3月30日 · Spleen tyrosine kinase (Syk) is involved in cellular adhesion and also in the activation and development of hematopoietic cells. Syk activation induced by genomic rearrangement has been linked to certain T-cell lymphomas, and Syk inhibitors have been shown to prolong survival of patients with B-cell …

To address the potential contribution of autophosphorylation and to understand if LYN is capable of phosphorylating Tyr-323 and Tyr-525/Tyr-526, we generated a K402R “kinase dead” mutant SYK (SYK-KD), which is <4% as active as WT SYK in the Omnia assay (data not shown).