ATM-associated signalling triggers the unfolded protein ... - Nature
2020年7月14日 · Upon ER stress, 293T cells may respond the stress by means of decreasing phosphorylated AKT and simultaneously by increasing unphosphorylated AKT in order to effectively suppress the cell ...
Targeting AKT1-E17K and the PI3K/AKT Pathway with an Allosteric AKT …
As a critical component in the PI3K/AKT/mTOR pathway, AKT has become an attractive target for therapeutic intervention. ARQ 092 and a next generation AKT inhibitor, ARQ 751 are selective, allosteric, pan-AKT and AKT1-E17K mutant inhibitors that potently inhibit phosphorylation of AKT.
Functional characterization of lysosomal interaction of Akt
2018年6月5日 · In 293T cells transfected with Akt, VRK2, and Phafin2, we observed Akt interaction with Phafin2 and VRK2, but no interaction between Phafin2 and VRK2 was detected (Fig. 2e). This observation...
PTEN suppresses tumorigenesis by directly dephosphorylating Akt …
2021年7月12日 · Here, we find that PTEN interacts with Akt and dephosphorylates Akt1 at S473 and T308 sites, thus negatively regulates Akt1 activity by short-circuiting the classical PTEN-PIP3-PDK1-Akt...
Modulation of Akt kinase activity by binding to Hsp90 | PNAS
2000年9月19日 · To examine the second possibility that the Akt binding to Hsp90β would affect PP2A-mediated Akt dephosphorylation, we examined the change of phospho-Akt level in 293T cells expressing 1–309 Akt after incubation with the PI(3)K inhibitor LY294002.
Akt-Mediated Phosphorylation of EZH2 Suppresses Methylation ... - Science
2005年10月14日 · Akt interacts with and phosphorylates EZH2 on Ser 21. (A) Coimmunoprecipitation assay of EZH2 and Akt. Lysates from 293T cells transfected with the indicated plasmids were immunoprecipitated with antibodies against HA or Myc. Whole-cell lysate (lysate) was also subjected to Western blot analysis to examine the expression of EZH2 and Akt.
AKT mediates actinomycin D-induced p53 expression - PMC
Thus, upon activation by ActD, AKT becomes an inducer of p53 tumor suppression instead of being a survival factor, as consistently shown in human embryonic kidney cell lines (293 and 293T), human hepatoma cell line (HepG2), and mouse hepatoma cell line (Hepa-1c1c7).
Molecular Imaging of Phosphorylation Events for Drug Development …
2008年12月2日 · Treatment of nude mice bearing 293T/AST xenografts with perifosine led to a 2-fold increase in complemented FL activities compared to that of 293T/ASA xenografts. Our system was used to screen a small chemical library for novel modulators of Akt kinase activity.
CK2 functionally interacts with AKT/PKB to promote the β-catenin ...
2011年7月7日 · We studied in HEK-293T cells, the effect of CK2-dependent hyperactivation of AKT on cell viability, as well as analyzed β-catenin subcellular localization and transcriptional activity and survivin expression.
FABD结构域缺失削弱293T细胞BCR/ABL癌蛋白抗凋亡能力
[摘要] 目的 通过转染野生型pAd-Bcr/Abl及缺失突变型pAd-BCR/ABL-ΔFABD于293T细胞中，探讨FABD结构域对Bcr/Abl癌蛋白抗凋亡能力的影响及相关机制。 方法 293T细胞分别转染野生型pAd-Bcr/Abl及缺失突变型pAd-Bcr/Abl-ΔFABD质粒，并设置Blank组和Adtrack组作为对照，分别培养24、48、72 h后，采用流式细胞术...