2014年11月13日 · JAK3 mutants unable to transform Ba/F3 cells did not cause JAK1 or JAK3 phosphorylation in 293T cells and were predicted as “neutral” variants using PROVEAN 22 analysis (supplemental Figure 2), supporting evidence that the R925S and E1106G are nontransforming passenger mutations.

2009年1月27日 · Western blot analysis of JAK1 in 293T cells showed that the E966V and Del (966–989) variants displayed weak JAK1 auto-phosphorylation, comparable to wild type JAK1, and in contrast to the strongly phosphorylated R724H mutant (Figure 1F).

2010年12月1日 · We assessed activation and downstream signalling of these transforming JAK1 mutants by expressing them in HEK-293T cells. Expression of JAK1-WT causes very little activation-loop phosphorylation (Figure 2A, lane 2), as measured using an antibody that is specific for JAK1 when phosphorylated at the activation-loop tyrosine residues Tyr 1034 and ...

2023年11月25日 · Here, we revealed that upregulated methyltransferase-like 3 (METTL3) in colorectal cancer exerted both methyltransferase activity-dependent and -independent functions in gene regulation. METTL3...

2014年12月6日 · Our results show that JAK1 is an essential kinase for most JAK3 mutants, and that the majority of JAK3 mutants are sensitive to JAK3 and JAK1 specific inhibitors such as tofacitinib and ruxolitinib. However, some JAK3 mutants do show resistance to these inhibitors, which will need to be taken into account when trials are initiated for the ...

2013年8月21日 · Here, we have shown using co-expression in 293T cells that while SOCS5 can specifically interact with all four JAKs it selectively inhibits the autophosphorylation of JAK1 and JAK2. The interaction is likely to be mediated by the identified, conserved JAK interacting region (JIR) in the SOCS5 N-terminus, whilst the inhibition appears to require ...

Here, we studied the ability of JAK1 V658F and A634D to activate the Janus kinase (JAK)/STAT pathway upon ectopic expression in HEK293 cells alone or together with the other components of the interleukin-9 receptor complex (IL-9Rα, γc, and JAK3).

2022年5月5日 · METTL3 mediated m 6 A modification on Jak1 mRNA in TIMs, the m 6 A-YTHDF1 axis enhanced JAK1 protein translation efficiency and subsequent phosphorylation of STAT3. Lactate accumulated in tumor microenvironment potently induced METTL3 upregulation in TIMs via H3K18 lactylation.

JAK3 假激酶突变体依赖 Jak1 激酶活性进行细胞转化，而 JAK3 激酶域突变体可以以不依赖 Jak1 激酶的方式转化细胞。 293T 细胞中 IL7 受体信号复合物的重建表明 JAK3 突变体需要受体结合来介导下游 STAT5 磷酸化。

2000年5月19日 · To explore the role of JAKs in IGF-I-induced STAT3 activation, we examined the tyrosine phosphorylation of transiently expressed JAK1 or JAK2 in 293T cells in response to IGF-I stimulation of endogenous IGF-IR.