293T-LRP6-beta-propeller-3-4-MESD-Middle-Cell-Line - Kyinno Bio
293T-LRP6-beta-propeller-3-4-MESD-Middle cell line was generated using a lentiviral vector expressing the human LRP6 sequence. Figure 1: Characterization of human LRP6 …
Primary cilia are WNT-transducing organelles whose biogenesis is ...
2023年1月23日 · We carried out live cell imaging of 293T cells co-transfected with LRP6-EGFP and pArl13b-mKate2 to examine the kinetics of LRP6 localizing to cilia upon serum starvation.
Reporter gene HEK 293 cells and WNT/Frizzled fusion proteins ... - PubMed
The chimera WNT3aFZD4 efficiently induced β-catenin-mediated luciferase activity. This activity was increased 40-fold compared with basal when LRP6 was stably cotransfected, proving that …
Kremen2 modulates Dickkopf2 activity during Wnt/lRP6 …
2003年1月2日 · Here we show that Kremen2 (Krm2) regulates Dkk2 activity during Wnt signaling. In human 293 fibroblasts transfected dkk2 activates LRP6 signaling. However, co-transfection …
Truncated mutants of the putative Wnt receptor LRP6/Arrow can …
2004年4月5日 · We have investigated this issue through a structure–function analysis of Frizzled and LRP proteins that have been implicated in Wnt1 signaling. Consistent with other reports, …
LDL-receptor-related protein 6 is a receptor for Dickkopf proteins
2001年5月1日 · We found that alkaline phosphatase (AP)-tagged Dkk1 (Dkk1–AP) binds effectively to 293T cells transfected with LRP6 (Fig. 1e). This interaction is specific, as …
R-Spondin1 regulates Wnt signaling by inhibiting internalization of LRP6
2007年9月9日 · Here we show that Rspo1 regulates Wnt signaling by modulating levels of LRP6 on the cell surface, through inhibition of DKK1-dependent internalization of LRP6. To further …
LRP6 Knockout cell line (293T) | Ubigene
The LRP6 Knockout cell line (293T) is constructed based on (1) the optimal design of the Red Cotton System, (2) CRISPR-U™ technology with efficiency 10-20 times higher than traditional …
Analysis of Endogenous LRP6 Function Reveals a Novel Feedback …
2007年8月13日 · In the present study, using a new, highly sensitive anti-LRP6 monoclonal antibody (MAb), we demonstrate that endogenous LRP6 is posttranslationally modified by …
Kremens, Dkk1 and LRP6 form a ternary complex.a–h, 293T cells …
Coexpression in 293T cells of luciferase-tagged LRP6 with YFP-tagged-krm1 or -2 results in a basal BRET signal which is enhanced by dkk1 and -2 but not by dkk3 (Fig. 4i).