TGF-β Reporter HEK 293 Cells - InvivoGen
HEK-Blue™ TGF-β cells were generated by stable transfection of the human embryonic kidney HEK293 cell line with a Smad-inducible secreted embryonic alkaline phosphatase (SEAP) reporter. The binding of TGF-β to its receptor triggers a signaling cascade leading to the formation of a Smad2/Smad3/Smad4 complex.
Cripto Binds Transforming Growth Factor β (TGF-β) and Inhibits TGF …
2006年10月9日 · Cripto binds TGF-β1 in the presence of TβRII and reduces cross-linking of TGF-β1 to TβRI. 293T cells were transfected with empty vector or the indicated constructs, labeled with 125 I-TGF-β1, and then subjected to covalent cross-linking and immunoprecipitation (IP) with an antibody directed against TβRII (A and B) or TβRI (C) as ...
Latent TGF-β structure and activation - Nature
2011年6月15日 · TGF-β assays used 293T cells stably transfected with α v β 6 integrin and transiently transfected with mutant human proTGF-β1, then co-cultured with indicator cells expressing a luciferase ...
The type I TGF-β receptor engages TRAF6 to activate TAK1 in a
2008年8月31日 · To elucidate the molecular mechanisms for TGF-β-induced activation of TAK1, we performed in vivo ubiquitylation assays in human embryonic kidney (HEK) 293T cells transfected with TAK1 and...
Generation of a TGF-b reporter cell line. (A) Schematic diagram of ...
(B) Representative luciferase response of 293T-caga cells to recombinant TGF-b. 293T cells were transduced with the pSMPUW- (caga) 12-Luc vector, and cultured in the presence of recombinant...
USP15 stabilizes TGF-β receptor I and promotes oncogenesis
2012年2月19日 · Our results show that USP15 regulates the TGF-β pathway and is a key factor in glioblastoma pathogenesis. TGF-β is a cytokine with a key role in tissue homeostasis and cancer. In advanced...
GDF9 activates the Smad3 signaling pathway in HEK-293T cells. HEK-293T ...
We found that the human embryonic kidney cell line HEK-293T was very responsive to GDF9, as measured by induction of the CAGA- luciferase reporter activity (P 0.01 for GDF9 vs. control; Fig. 1A)....
293-TGFß Res 细胞株（DMEM） (Cat. No.:XCC03-1)
本产品提供稳定表达报告基因的细胞株。 可以提供从抗体发现、信号通路阻断和细胞功能检测等过程，为TGF-β靶点的临床前体外研究提供解决方案。
TGFβ通路报告基因检测 - 知乎
本检测是利用稳定转染pCAGA12-TA-Luc质粒的HEK293或者A549细胞系，评价药物对TGF-β依赖的CAGA12转录激活的抑制作用。 加入 TGF-β1 能够诱导CAGA12的转录激活作用，HEK293或者A549/CAGA12-Luc细胞 萤火虫萤光素酶 的活性就会提高，加入TGF-β I 型受体 ALK5、ALK4 和 ALK7 的抑制剂A83-01能抑制TGF-β诱导的CAGA12转录激活作用。 参考文献. Derynck R, Turley SJ, Akhurst RJ. TGFβ biology in cancer progression and immunotherapy. Nat. Rev. Clin. …
TGF-β中和抗体药效学评价-爱康得生物科技（苏州）有限公司
TGF-β（transforming growth factors-β， 转化生长因子 TGF-β）是一种多向性、多效性的细胞因子，通过开启细胞表面的受体信号级联转导途径调节细胞的增殖、分化、迁移和凋亡，对胞外基质的合成、创伤的修复、免疫系统的功能等有重要的调节作用。 图示说明：TGF-β在抑制肿瘤适应性免疫中扮演重要角色. TGF-β的信号传导通路有两种: TGF-β-Smad信号通路和Non-Smad信号通路。 其中Smad信号通路是TGF-β产生生物效应的主要途径。 TGF-β在细胞表面与II型受体(TGF …