2017年3月14日 · We expressed TREX1-V5 and Cyclin B1-Myc 6 in 293T cells enriched in mitosis by nocodazole treatment and pulled down TREX1 with anti-V5 antibody beads. Indeed, we found cyclin B1 to co-immunoprecipitate with TREX1 (Figure 1 G).

2024年12月13日 · Here, we report human TREX1 mosaicism causing organ-limited RVCL in the retina, as well as a gene therapy to synthetically create TREX1 mosaicism as a potential treatment for RVCL.

2015年9月15日 · Retroviruses were packaged in 293T cells and used for infection of Trex1−/− MEFs followed by selection with blasticidine (Sigma). For shRNA knockdowns, Trex1−/− MEFs were transduced with lentiviruses expressing specific shRNA (pLKO system, Addgene) and selected with puromycin.

In this study, we show that TREX1 modification by ubiquitination is controlled by a highly conserved sequence in the CTR to affect cellular localization. Transfection of TREX1 deletion constructs into human cells demonstrated that this sequence is required for ubiquitination at multiple lysine residues through a “non-canonical” ubiquitin linkage.

Representative Western blot of transfected TREX1 in 293T cells (lanes 1–2, 9–10) and untransfected EBV transformed lymphocytes from normal controls and RVCL mutation carriers.

2014年6月15日 · Second, HEK STING cell lines (10) (HEK 293T cell lines expressing fluorescently labeled STING) that lack functional gap junction communication (Connexin43 and Connexin45 double-knockout cells) were unresponsive toward the TREX1-deficient MEFs, yet showed normal induction of ISGs when transfected with the RIG-I stimulus 5′-triphosphate RNA ...

(C) Immunoblot analysis of TREX1 interactions with RPN1 and DDOST. 293T cells were transfected with plasmids indicated on top. Immunoprecipitation (IP) was performed with anti-Myc antibody.

V5-tagged TREX1 serine mutants were expressed in 293T cells, immunoprecipated with V5 beads, and subjected to in vitro DNase assay as in Figure 3. All serine mutants remain DNase active similar to wild type TREX1. D200H is known to be DNase-dead. Figure S3. TREX1 serine mutants localize to the ER properly. Related to Figure 3.

2017年3月14日 · TREX1 has a DNase-independent function in the C terminus that regulates the ER oligosaccharyltransferase (OST) activity. Kucej et al. found that TREX1 is phosphorylated during mitosis at Serine-261 in the C terminus. Mitotic phosphorylation disrupts TREX1 interaction with the OST complex without affecting its DNase activity.

2018年10月10日 · Two major bands at 37 and 32 kDa were detected in 293T cells (Fig. 1 B) along with an unidentified light band at 27 kDa, intermittently seen on WBs, which may be a fragment of TREX1 as reported previously 17.