Multicistronic or bicistronic vectors contain IRES and/or 2A peptides to allow for coexpression of multiple genes from a single transcript.

2017年5月19日 · To facilitate rapid and convenient cloning in the current study and future applications, we built a set of cloning vectors that contain three 2A sequences and four pairs of …

Mammalian expression vector for tagging proteins with an N-terminal FLAG epitope. For other reading frames, use pCMV-Tag 2B or pCMV-Tag 2C.

2012年2月1日 · This article describes the design and construction of 2A peptide-linked multicistronic vectors, which can be used to express multiple proteins from a single open …

2 天之前 · Not sure whether to use an IRES or a 2A linker in your polycistronic expression cassette? VectorBuilder's custom cloning experts are here to help!

2025年3月12日 · VectorBuilder offers both read-through and non-read-through linkers including 2A peptides and IRES for connecting multiple ORFs in polycistronic vectors.

All 2-series vectors work as single-expression vectors, as well as transfer vectors for our polycistronic system. The LIC cloning site is flanked by 5 pairs of restriction sites, so that your …

The pros and cons of 2A co-expression technology are considered below, followed by a simple example of a "how to" protocol to concatenate multiple genes of interest, together with a …

2018年4月19日 · This is followed by an illustrative “how to” example that describes the generation of a 2A peptide-based vector that directs coexpression of the therapeutic human (IDUA) gene …

2A is an oligopeptide sequence that mediates a ribosome “skipping” effect and can mediate a co-translation cleavage of polyproteins. These sequences are widely distributed from insect to …