
Hyperthermia-triggered drug delivery from iRGD-modified …
2016年12月10日 · 4T1 mammary carcinoma cells, MCF-7 human breast adenocarcinoma cell and Human Umbilical Vein Endothelial Cells (HUVECs) were purchased from ATCC. 4T1 and MCF-7 were cultured in DMEM medium with 10% fetal bovine serum (FBS), 100 U/ml penicillin, 100 μg/ml streptomycin and 2 mM l -glutamine.
HUVEC, 4T1, and U87 cells viability following exposure to …
Figure 1 (a) shows that the VGB inhibited HUVEC, 4T1 and U87 cell proliferation in a dose-and time-dependent manner. Results indicated that VGB inhibited cell proliferation at concentration of...
Synergic antitumor effect of SKLB1002 and local hyperthermia in 4T1 …
2012年12月21日 · In the present investigation, we aim to prove that combination therapy of SKLB1002 with hyperthermia plays a synergy as an antitumor agent in solid tumor. In this study, we analyzed their synergetic inhibitory action on human umbilical vein endothelial cells (HUVEC), murine mammary cancer 4T1, murine colon carcinoma CT26 in vitro.
A peptide mimicking the binding sites of VEGF-A and VEGF-B
2018年12月18日 · Based on in vitro and in vivo studies, VGB4 potently inhibited proliferation, migration and tube formation of human umbilical vein endothelial cells (HUVECs), as well as 4T1 mammary carcinoma...
【整理总结】人脐静脉内皮细胞(HUVEC)常见问题贴(2014年9 …
很多站友会养人脐静脉内皮细胞(HUVEC),作为初来乍到的科研新手,都有这样一种惆怅的感受:“细胞代代无穷已,江月年年只相似”(改编自唐代诗人张若虚的《春江花月夜》),所以如何处理那些似曾相似的问题,
Evaluation of Microwave Ablation in 4T1 Breast Tumor by a Novel …
2021年7月20日 · The specific targeting ability in vitro was tested by HUVEC and 4T1 cells in two ways: qualitative observation by CLSM and quantitative detection by flow cytometer (FCM; Beckman Coulter, Brea, CA, USA).
Double enhancement of immunogenic cell death and antigen presentation ...
2021年8月1日 · The murine breast cancer cell line 4T1, human umbilical vein endothelial cells line HUVEC and mouse mononuclear macrophage leukemia cell line RAW264.7 were obtained from Southwest Jiaotong University (Chengdu, China), luciferase labeled mouse breast cancer cells (Luc-4T1) cells were obtained from Sichuan University (Chengdu, China). 4T1, Luc ...
Profiling target engagement and cellular uptake of cRGD …
Our experimental set-up included the incubation of four cell lines/primary cells (Α431, HUVEC, activated HUVEC, 4T1) that are well-known to display different α v β 3 integrin expression levels (Fig. S1 – α v β 3 integrin receptor-target of cRGD ligand) with all four CCPM variations (0, 1, 3.6, and 5 mol% cRGD), at various temperatures (4 ...
HUVEC-T1(SV40T转化人脐静脉内皮细胞)培养条件及注意事项
HUVEC-T1内皮标记vWF、CD31、CD34均阳性,可结合凝集素,电镜下可见紧密连接和W-P小体,Matrigle上可形成管型。 不同代数的HUVEC-T1细胞核型正常稳定(P12、P13、P19、P38),裸鼠体内接种不成瘤(P9和P33)。
新资源:SV40T转化人脐静脉内皮细胞HUVEC-T1
2013年3月7日 · 中国医学科学院基础医学研究所北京协和医学院基础学院细胞资源中心,原代分离培养人脐静脉内皮细胞,通过感染SV40的大T和小T抗原,建立了可体外连续传代的人脐静脉内皮细胞系PUMC-HUVEC-T1。
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