2014年2月16日 · The 5' and 3' mean "five prime" and "three prime", which indicate the carbon numbers in the DNA's sugar backbone. The 5' carbon has a phosphate group attached to it and the 3' carbon a hydroxyl (-OH) group. This asymmetry gives a DNA strand a "direction".

2012年1月5日 · If one or more nucleotide is missing in one strand, repair of the missing nucleotide would be impossible for 3' to 5' synthesis, because no 5'-triphosphate is present. On the other hand, 5' to 3' synthesis does not require a 3'-triphosphate present at the repair site. This is important. That is 3' to 5' synthesis does not allow nucleotide repair.

2014年2月13日 · Before the start codon AUG the mRNA contains a regulatory, untranslated region, the so-called 5'UTR. The end of the 5'UTR of eukaryotes contains the so called methylguanosine cap (and is only present in mature mRNA), in proaryotes this is done by the Shine-Delgano sequence. Both are recognized by the ribosome.

2021年4月21日 · Both the sugar and the nucleobase have Carbons and those carbons are called with numbers. The nucleobase gets the first "round" of names: Carbon 1, Carbon 2, etc. while the sugar gets the prime ones: Carbon 1 prime (that is, another Carbon 1), Carbon 2 prime (another Carbon 2), etc. So the 5' means Carbon 5 from the sugar in the nucleotide.

2016年2月25日 · DNA polymerase III adds nucleotides in the $5^\prime \rightarrow 3^\prime$ direction because it can only add nucleotides to the $3^\prime$ end of the previous nucleotide. This is why it requires a primer. However, does DNA polymerase I operate by the same criterion?

2016年4月11日 · In fact, approximately 35% of 5′-UTR precursors contain introns, not present in the mature mRNA. 5′-capping of eukaryotic mRNA. The vast majority of eukaryotic mRNAs have a cap at their 5′-end, produced by the addition of a guanosine nucleotide in a 5′-5′ pyrophosphate linkage to the 5′-residue (usually A) of the pre-mRNA.

2014年4月22日 · Transcription always proceeds in the direction 5' (5-prime) to 3' (3-prime) on the coding strand of DNA. Binding of both transcription factors and RNA polymerase to DNA depends on sequence motifs in the DNA.

Every chart of palindromic restriction enzymes I've seen lists their restriction sites from 5' to 3', something like this: EcoR1 cuts GAATTC between the G and A: 5' NNNGAATTCNNN 3' --> 5' NNNG____ AATTCNNN 3' 3' NNNCTTAAGNNN 5' --> 3' NNNCTTAA____ GNNN 5' Will restriction enzymes match and cut a site if it's running antiparallel?

2018年4月29日 · It seems that the promoter regions are written from the 5' to 3' direction. My lecture notes, for example, give the -35 and -10 promoter regions sequences like so: Wikipedia seems to agree, on their article about promoters. However, RNA polymerase transcribes in the 3' to 5', as in it reads the template strand in the 3' to 5' direction.

2021年1月23日 · DNA ligase catalyzes the formation of a bond between 3’-OH and 5’-PO 4 of its nucleic acid substrate. (See Lehnman, 1974) The ligation bond will be formed when the proper ends are close together, allowing the ligase to act. Lets redraw your diagram and add the 5’ and 3’ designations to it. I think this may help clear out some points.