In this chapter, a description of the benefits and uses of 6xHis-fusion proteins as well as a detailed method for performing a 6xHis-pulldown assay are described. Fusion-protein tags provide a useful method to study protein-protein interactions. One widely used fusion tag is hexahistidine (6xHis).

The DNA sequence specifying a string of six to nine histidine residues is frequently used in vectors for production of recombinant proteins. The result is expression of a recombinant protein with a 6xHis or poly-His-tag fused to its N- or C-terminus.

2014年10月20日 · The 6xHis tag, also known as polyhistidine tag, His6 tag and/or hexa histidine tag, is an amino acid motif consisting of at least 6 histidine residues fused to the carboxyl (C-) or amino (N-) terminus of a target protein in transfected cells.

2019年6月12日 · 6xHis标签，也称为多组氨酸标签（polyhistidine ），His6标签或hexa组氨酸标签，这是一种在转染的细胞中目标蛋白的C端或N端上由至少6个组氨酸残基链接上所组成的氨基酸序列，它的序列如下所示：

Recombinant proteins containing a His-tag can be purified by Ni-NTA chromatography which is based on the interaction between a transition Ni2+ immobilized on a matrix and the histidine side chains. Abb. 1. Capture of His-tag proteins by metal-chelate affinity matrices relies on …

In the earliest works of one-step purification of polyHis tagged target proteins by IMAC, 6xHis was successfully utilized to purify recombinant protein constructs to 95% purity in high yield.

2022年7月14日 · 6xHis标签，也称为多组氨酸标签（polyhistidine ），His6标签或hexa组氨酸标签，这是一种在转染的细胞中目标蛋白的C端或N端上由至少6个组氨酸残基链接上所组成的氨基酸序列，它的序列如下所示：

A handbook for high-level expression and purification of 6xHis-tagged proteins pdf 600KB English Format File size Language Download Get Adobe Reader Contact QIAGEN . Global contacts. Technical Service; Customer Care . Related products. Show All ...

2023年7月28日 · Polyhistidine tags are peptides consisting of 6 or more consecutive histidine residues (6XHis) that are commonly used for protein purification by immobilized metal affinity chromatography...

The 6xHis/Ni-NTA system is a fast and versatile tool for the affinity purification of recombinant proteins and antigenic peptides. It is based on the high-affinity binding of six consecutive histidine residues (the 6xHis tag) to immobilized nickel ions, giving a...