本試劑盒採用TUNEL (TdT mediated dUTP Nick End Labeling)法，應用末端脫氧核糖核苷酸轉移酶 (TdT)在凋亡細胞斷裂的DNA的3′-羥基 (3'-OH)末端催化摻入螢光素-12-脫氧三磷酸尿苷 (FITC-12-dUTP)。 FITC-12-dUTP標記的DNA可以用螢光顯微鏡直接觀察或者用流式細胞儀定量。 圖1. 小鼠肝臟組織。 組織切片經DNase I處理後TUNEL染色。 A-C分別為BrightGreen染色後於紫外光 (460nm)下照射1min、3min、6min後拍照。 D-F分別為FITC染色後於紫外光 (460nm)下照 …

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