2021年11月1日 · Developing novel immunotherapy ADCs integrated with cytotoxicity and immunostimulation is a promising research project. A bifunctional PD-L1-ADC was developed based on the use of endocytic PD-L1 antibody Atezolizumab, exhibiting potent cytotoxicity (EC50 = 9.75 ∼ 11.94 nM) and immune activation effect.

2021年1月1日 · A431 tumor cells were labeled with 100 nM Alexa Fluor 546 (546)-conjugated anti-EGFR mAb (HuML66) in the presence (namely A1) or absence (A2) of cetuximab for 1h at room temperature respectively. 488-Labeled T cells were then co-cultured with 546-labeled A431 cells as the following formats T1+A2 or T2+A1 in PBS for 30min at room temperature ...

In this assay, the IC50 of PD-L1xEGFR for inhibiting the binding of a competing APC-labeled PD-L1 mAb to A431 cells was calculated to be 0.013 µg/ml which was ∼140 times lower than the IC50 calculated for PD-L1xMock.

2024年12月28日 · We first used flow cytometry to isolate A431 cells and tumor-associated macrophages from the tumor tissues, and then used western blotting to detect the expression of p-PI3K, p-AKT, and PD-L1...

2017年4月1日 · Detection of EGF-induced PTMs for PD-L1. (A) Serum-restricted A431 cells were either unstimulated (UT) or stimulated with EGF for 1 hour prior to lysis with BlastR lysis buffer. WCL was analyzed for PD-L1 levels (lanes 1 and 2). Ubiquitin binding beads (UBA01) were used to IP ubiquitinated proteins (lanes 3 and 4).

One study revealed that EGF stimulation increased tyrosine phosphorylation and acetylation of PD-L1 in A431 cells . Another study reported that overexpression of p300 enhanced PD-L1 acetylation, whereas depletion of p300 or p300 inhibitor A485 reduced the acetylation of PD-L1 . Moreover, Lys263 site was identified as the major acetylation site ...

2021年2月14日 · In the present study, we found a positive correlation between PD-L1 expression and MET phosphorylation in lung cancer and melanoma cell lines. PD-L1 inhibition led to a decrease in MET phosphorylation, while PD-L1 induction by IFN-γ resulted in a PD-L1-dependent increase of MET phosphorylation both in vitro and in vivo.

New bispecific antibody (bsAb) formats combining the ICB effect and a direct action on cancer cells could improve the efficacy of current immunotherapies. Here, we report the development of a PD-L1/EGFR symmetric bsAb by fusing a dual-targeting tandem trimmer body with the human IgG1 hinge and Fc regions.

PD-L1xEGFR for EGFR-directed PD-1/PD-L1 blockade was assessed by replacing CHO.PD-L1/CD3 cells in the standard PD-1/PD-L1 Blockade Bioassay by A431 cells (PD-L1 C /

2022年7月19日 · Atezolizumab has proved to induce PD-L1 internalization in PD-L1 positive cell lines, SK-MES, PC-9 and Calu-1 (lung cancers), MDA-MB-231 (triple negative breast cancer), MC-38 (murine colon adenocarcinoma), A431 (epidermoid carcinoma) and PD-L1 transfected CHO-K1 (Chinese hamster ovary).