2022年5月23日 · Transfection of A549 and H2052 cells with SFD-p16 significantly increased p16 mRNA expression levels and decreased cell proliferation in both cell lines. Similar results were obtained with...

2005年12月20日 · Here we show that cationic liposome-mediated gene transfection of INK4a/ARF into lung cancer cell line A549, in which the INK4a/ARF locus was lost, suppressed the growth and induced apoptosis.

为构建稳定表达外源性抑癌基因p16的肺癌A549细胞株,用脂质体介导的基因转染方法,借助真核质粒表 达载体(pcDNA3),将抑癌基因p16转移入此基因缺失的人肺癌细胞株A549细胞中,经G418筛选,获得稳定表达的细胞克隆,用逆转录聚合酶链反 应 (RT-PCR)及免疫组织化学鉴定p16 ...

2005年12月20日 · Here we show that cationic liposome-mediated gene transfection of INK4a/ARF into lung cancer cell line A549, in which the INK4a/ARF locus was lost, suppressed the growth and induced apoptosis.

2023年6月21日 · A549 and IMR-90 cells were transduced with p16 -overexpressing adenovirus, and treated with palmitic and oleic acids (P&O) to induce steatosis in vitro. We found that long-term HFD promoted the expression of p16 and the increase of senescent cells in the lung.

2012年12月1日 · Results from flow cytometric analysis demonstrated that PRMT6 overexpression counteracted the cell cycle arrest at G1 phase induced by wild-type p16 in A549 cells. We also provided evidence that PRMT6 was able to interact with p16, and that the intensity of p16-CDK4 association was reduced upon PRMT6 overexpression.

The present study indicated that the mRNA changes regulated by the methylation of RASSF1A, MGMT, p16, RARβ might be the possible reason for A549＇s sensitivity to cisplatin. As a potential biomarker for the sensitivity to cisplatin, the methylation panel of RASSF1A, MGMT, p16, RARβ in lung cancer is deserved to be further investigated in clinic.

p16 gene promoter region of DNA methylation status were detect by MSP method in 5-Aza-CdR processing A549 cells, the expression of p16 in A549 lung cancer cell and effect of 5-Aza-CdR were detect...

Exogenous p16(ink4a) gene was transfected by lipofectin into human lung cell line A549, in which p16(ink4a) gene was homozygously deleted. The expression of p16(ink4a) mRNA and protein was detected by RT-PCR and immunocyto-chemistry, respectively.

To assess the role played by p16 gene expression in the radiosensitivity of human lung cancers, we transferred exogenous p16 genes into p16-deficient H460 and A549 lung adenocarcinoma cell lines and compared the cell survival curve in vitro after irradiation.