2017年4月7日 · That necroptosis was activated in cells experiencing Ca ++, and K + dysregulation was verified by detection of pMLKL in ionomycin- and nigericin-challenged A549 cells using fluorescent...

2024年10月3日 · DNA damage-inducible transcript 3 (DDIT3) is a well-known transcription factor that regulates the expression of apoptosis-related genes for promoting apoptosis during endoplasmic reticulum stress....

2018年4月11日 · Herein, we show that respiratory epithelial cells (REC) undergoing bacterial pore-forming toxin (PFT)-induced necroptosis simultaneously experienced caspase activation independently of RIPK3. MLKL...

2024年7月12日 · We then assessed the presence of pMLKL in A549-ACE2-TMPRSS2 cells at 24 HPI and found pMLKL in the cells infected with either Delta or Omicron (fig. S7H). As anticipated, the percentage of pMLKL in the infected cells was significantly higher with Delta infection than with Omicron infection (fig. S7I; Delta versus Omicron, P value: <0.0001).

2020年3月19日 · Here, we show that replicating IAV generates Z-RNAs, which activate ZBP1 in the nucleus of infected cells. ZBP1 then initiates RIPK3-mediated MLKL activation in the nucleus, resulting in nuclear envelope disruption, leakage of DNA …

2020年6月29日 · 作者又用NSA封住MLKL和膜互作的位点，发现NSA抑制了MLKL的寡聚、膜转位、pMLKL聚集成hotspots，最终抑制了膜损伤，且NSA加入的越早抑制作用越强，暗示MLKL和膜的互作对于MLKL诱导程序性坏死很重要

2019年10月1日 · Expression of osteopontin (OPN) and endoplasmic reticulum (ER) stress, OPN and receptor-interacting protein (RIP)1, and phosphorylated mixed lineage kinase domain-like protein (pMLKL) in A549 cells after tumour necrosis factor-alpha (TNF-α) treatment. A549 cells were challenged with TNF-α or OPN for 24 h.

2018年2月1日 · In this study, we show that apart from apoptosis, cisplatin induces necroptosis in A549 cells. The alleviation of cell death by two necroptosis inhibitors-necrostatin-1 (Nec-1) and necrosulfonamide (NSA), and the phosphorylation of mixed lineage kinase domain-like protein (MLKL) at serine 358, suggest the involvement of receptor-interacting ...

ROC curve revealed a high predictive value of pMLKL score at T1 (AUC 0.70) and the ratio of pMLKL score at T1 and T0 (pMLKL-index, AUC 0.82) for EAD. Liver grafts with a high pMLKL index...

2020年10月16日 · Mixed lineage kinase domain-like pseudokinase (MLKL) is considered to be the key mediator of necroptosis. After necroptosis initiation, MLKL together with RIP1 and …