Ultrasound-targeted microbubble destruction enhances gene transduction ...
Adeno-associated virus (AAV) is a common vector utilized in gene therapy. The NIH/3T3 cell line, which is a potential induced pluripotent stem (iPS) cell type, was identified to be a less-permissive cell type to AAV due to its defective endosomal ...
Assessment of Adipocyte Transduction Using Different AAV …
2024年9月18日 · Results: AAV2/6 demonstrated the highest transduction efficiency in 3T3-L1 preadipocytes, as it was 1.5–2-fold more effective than AAV2/5, and AAV2/8 in the range of viral concentrations from 2 × 10 4 to 1.6 × 10 5 VG/cell. AAV2/5 and AAV2/8 showed transduction efficiencies similar to each other.
Adeno-Associated Virus Type 2-Mediated Gene Transfer: Altered …
Hypothetical model comparing intracellular trafficking of AAV in 293 (A) and NIH 3T3 (B) cells. AAV binds and enters the early endosomes of both cell types efficiently (a).
Assessment of Adipocyte Transduction Using Different AAV …
2024年9月18日 · Results: AAV2/6 demonstrated the highest transduction efficiency in 3T3-L1 preadipocytes, as it was 1.5-2-fold more effective than AAV2/5, and AAV2/8 in the range of viral concentrations from 2 × 10 4 to 1.6 × 10 5 VG/cell. AAV2/5 and AAV2/8 showed transduction efficiencies similar to each other.
High-Efficiency Transduction of Fibroblasts and Mesenchymal …
Single mutations in surface-exposed tyrosine residues improve the transduction efficiency of AAV2 vectors in murine NIH 3T3 cells. To develop the optimal AAV vectors for fibroblast and MSC gene transfer, we first tested the transduction efficiency of each of the seven (Y252F, Y272F, Y444F, Y500F, Y700F, Y704F, and Y730F) surface-exposed ...
Nat Commun：AAV特异性敲除脂肪细胞Tmem120a引发脂肪营养 …
2022年5月17日 · 用laminB1-Dam甲基化酶慢病毒转导3T3-L1细胞的野生型和Tmem120a敲低细胞系获得甲基化的脂肪细胞系，富集Dam甲基化DNA测序，来鉴定层相关结构域（LADs），研究基因的定位情况。并通过对Tmem120a特异性敲除小鼠、敲低Tmem120a和Tmem120b基因的稳定3T3-L1 shRNA细胞系等的相关 ...
Celastrol enhances AAV1-mediated gene expression in mice
2010年9月16日 · We have found that celastrol, a chemical from a traditional Chinese herb known to inhibit the proteasome activity, was able to enhance the transgene expression mediated by AAV1 in 3T3-L1...
【求助】求3T3-L1细胞慢病毒转染详细方案 - 丁香园论坛
首先在收到病毒液之前你需要把细胞状态调整好，查找文献看看3T3-L1细胞的感染复数是多大（即MOI值， MOI 是multiplicity of infection 的缩写，中文译为感染复数。
Cas12a-knock-in mice for multiplexed genome editing, disease
2 天之前 · The LbCas12a enzyme along with adeno-associated virus (AAV) ... able to efficiently deliver small RNA such as Cas9-tracrRNA in vitro to the mouse embryonic fibroblast cell line NIH/3T3 ...
腺相关病毒载体（AAV）在肺脏的研究及应用 - 知乎
研究中采用的 重组腺相关病毒 （Recombination adeno-associated virus,rAAV）载体是在非致病的野生型AAV的基础上改造的基因表达载体，具有种类多样、免疫原性低、安全性高、宿主范围广、扩散能力强、体内表达外源基因时间长等特点，已被广泛的应用在动物水平的基因 ...