Plasmid AAV GFP from Dr. Fred Gage's lab contains the insert eGFP and is published in Proc Natl Acad Sci U S A. 2002 Feb 19;99 (4):2320-5. Epub 2002 Feb 12. This plasmid is available through Addgene.

Encodes an N-terminal GFP, Myc-tag fused in-frame to an SV40 nuclear localization signal (NLS) and Cre recombinase (AAV-GFP/Cre). Expression is driven by a CMV promoter with a splice donor/acceptor sequence (SD/SA) and flanked by inverted terminal repeats (ITRs).

pAAV-GFP腺相关病毒包装对照质粒是一种携带有绿色荧光蛋白 (Green Fluorescent Protein, GFP)基因的病毒包装质粒，广泛用于基因转导实验的质控和效率评价。 在基因治疗研究或细胞生物学研究中，pAAV-GFP质粒常被用来验证AAV颗粒的包装、纯化及转导效率。 这个质粒包含了AAV的反向终结子 (ITRs)，这是AAV包装所必需的唯一病毒序列，同时还含有GFP报告基因。 ITRs夹持GFP基因，当与其他辅助质粒共转染生产细胞如HEK 293细胞时，即可产生含 …

AAV-mCherry-GFP-LC3B是碧云天自行研发的重组腺相关病毒，感染后能够在靶细胞中有效表达红色荧光蛋白mCherry、绿色荧光蛋白(GFP)和LC3B的融合蛋白，呈现明亮的红色和绿色荧光，可以用于细胞自噬的检测。

AAV-GFP报告基因表达系统方法基于AAV-GFP表达系统与样本孵育后在体外感染HEK293T后直接表达绿色荧光蛋白，利用流式细胞术检测细胞的感染效率。 利用公式抑制率（Inhibition）% =100- [（测试样本感染效率-当批平均空白样本感染效率）/（当批平均阴性质控感染效率-当批平均空白样本感染效率）]*100计算中和抗体对病毒感染的抑制率。 图三为AAV-GFP报告基因表达系统检测抗AAV中和抗体的原理图。 针对抗AAV2中和抗体我们可选用HEK293细胞进行检测，针对AAV8 …

2021年1月21日 · We evaluated the quantity of viral DNA from the serotypes AAV9, AAV6, and a mosaic AAV1&2, expressing green fluorescent protein (GFP) under the neuron-specific synapsin promoter (syn). AAVs were...

In this study, we examined potential pathogenic responses to injection of a recombinant AAV containing the marker gene, green fluorescent protein (AAV-GFP). To assess transduction stability, we quantified the number of GFP immunoreactive neurons over time. Furthermore, we examined the spread of GFP through CNS pathways.

2020年12月9日 · To validate our recent protocol, we present here a direct side-by-side comparison between vectors produced with either method in a series of in vitro and in vivo assays with a focus on transgene...

2025年3月10日 · For amplification and recovery of the AAV barcode (BC) region for NGS analysis, the barcoded region of AAV-CMV-GFP-BC-WPRE cassette was amplified from 50 ng of total genomic and AAV Testing Kit ...

2 天之前 · Interneuron-specific dual-AAV SCN1A gene replacement corrects epileptic phenotypes in mouse models of Dravet syndrome. John K. Mich https: ... GFP/empty vector (GFP, n = 11), or SCN1B/2B (β1/2, n = 13). All HEK293 transfections were performed along with an SCN1B/2B expression plasmid, at a ratio of 10:1. Medians displayed with data points.