Adeno-associated viruses (AAV) are small viruses that infect humans and some other primate species. They belong to the genus Dependoparvovirus, which in turn belongs to the family Parvoviridae.

AAV（腺相关病毒）的装载容量主要由其基因组长度限制，约为 4.7 kb（kilobases），这是AAV病毒颗粒可以容纳的DNA总长度，包括病毒的反向末端重复序列（ITR）和目标序列。

1997年4月21日 · Here we investigate the maximum amount of DNA which can be inserted into the AAV genome and still allow efficient packaging into an infectious virus particle. Altered wild-type AAV genomes were constructed with inserts, which increased in …

2021年2月4日 · Size exclusion chromatography (SEC) coupled to multiangle light scattering (MALS) offers a straightforward approach to comprehensively characterize AAV capsids.

Adeno-associated virus (AAV) vector genomes have been limited to 5 kilobases (kb) in length because their packaging limit was thought to be similar to the size of the parent AAV genome. Recent reports claim that significantly larger vector genomes ...

AAV（腺相关病毒）包装的大小主要由其基因组的最大容量决定。 一般来说，AAV的包装上限约为 4.7 kb （kilobase）。 这一限制包括了需要的病毒序列（如 ITRs，即反向末端重复序列），因此留给外源基因或载体设计的有效插入容量略小。 关键点：

1996年7月17日 · Our studies showed that the optimal size of AAV vector is between 4.1 and 4.9 kb. Although AAV can package a vector larger than its genome size, up to 5.2 kb, the packaging efficiencies in this large size range were sharply reduced.

2023年11月1日 · Adeno-associated virus (AAV) is the leading platform for in vivo gene therapy to treat numerous genetic diseases. Comprehensive analysis of the AAV particles is essential to ensure desired safety and efficacy. An array of techniques is required to evaluate their critical quality attributes.

Adeno-associated virus (AAV) consists of a non-enveloped protein capsid with diameter ~25 nm, packed with single-stranded DNA. With a history of over fifty years of study, AAV has become one of the most popular and well-characterized gene-delivery vectors for clinical applications.1,2.

2024年3月14日 · The present work studied the effect of the genome size on AAV production, purification, and thermostability by producing AAV2-GFP using suspension-adapted HEK293 cells via triple transfection with three different sizes of single-stranded AAV genome containing GFP consisting of 1.9, 3.4, and 4.9 kb (inverted terminal repeat [ITR] to ITR).