基于AAV7的病毒载体在小鼠模型中证明对骨骼肌细胞具有很高的转导效率，与AAV1所达到的效果相似，并且高于AAV2。 该血清型也被证明对鼠和人组织中的肝细胞具有强烈的趋向性。

The seventh serotype of AAV (AAV7) was first isolated in 2002 from NHP tissues, specifically, from Rhesus macaque monkeys [35, 129]. Its mechanisms of cell binding and cell entry are still unknown [52, 130], but it is established that this serotype does not bind heparin, or any other glycan in general [131].

2019年9月30日 · Immunofluorescence was used to detect immunostained GFP (red) expression in human hepatocytes immunostained for human serum albumin (green). a AAV3B, AAV7 or AAV8 vector transduction efficiency...

2024年9月26日 · We analyzed 133 serum samples from patients with NMDs and 76 serum samples from healthy individuals to identify promising viral vectors among 18 natural or engineered AAV variants based on low pre-existing seroprevalence of …

2024年12月12日 · AAV7 (4282L) (median NT 50: 179.0) induced substantially increased NAbs to RhAAV4282 compared with AAV7. These data suggest that the HRNE is also a partial neutralization epitope for RhAAV4282.

AAV9 had the best viral genome distribution and highest protein levels. The AAV7 protein and genome copy numbers were comparable to those of AAV9 in the liver. Most surprisingly, AAV4 showed the greatest number of genome copies in lung and kidney, and a …

2009年2月2日 · In this study, we obtained 888 human serum samples from healthy volunteers in 10 countries around the world. Samples were assayed for neutralizing antibodies to AAV1, AAV2, AAV7, and AAV8, as well as to a novel, structurally distinct AAV vector, rh32.33, in an in vitro transduction inhibition assay.

AAV7’s VR-VII is the shortest loop among all AAV serotypes with a 1 aa deletion compared to AAV1-AAV4, and AAV6-AAV13 and a 4 aa deletion compared to AAV5. AAV7 vectors were shown to result in high transduction efficiencies of the CNS and spinal cord after delivery into the cerebrospinal fluid or intravenously [63, 64].

In this paper, Shao et al. [9] compare rAAV serotypes in a humanized liver xenograft model and demonstrate that rAAV7 is actually superior in hepatocyte tropism to either rAAV8 or rAAV3B, as determined with hepatocytes from multiple different individual human donors.

2020年9月11日 · For human liver targeting, two major factors restrict effective AAV transduction after systemic administration of AAV vectors: human hepatocyte tropism and neutralizing antibodies (Nabs). In this study, we attempted to isolate AAV variants with the ability to transduce human hepatocytes and escape Nabs using a directed evolution approach in vivo.