2019年12月26日 · The high consistency between the proposed approach RCA-assisted CRISPR/Cas9 cleavage (RACE) and reverse transcription quantitative polymerase chain …

2025年2月21日 · To this purpose, a primer-regulated rolling circle amplification (RCA) strategy was ingeniously proposed. Briefly, the RCA primer, which was invalidated with 3′-inverted dT …

2023年12月20日 · Herein, a robust strategy based on the dephosphorylation switch DNAzyme-rolling circle amplification (RCA) circuit, termed DSD-RCA, is developed for highly sensitive …

2020年1月21日 · Herein, we developed a highly specific nucleic acid detection platform for simultaneous quantification of several EV-derived miRNAs in constant temperature by …

2025年2月21日 · To this purpose, a primer-regulated rolling circle amplification (RCA) strategy was ingeniously proposed. Briefly, the RCA primer, which was invalidated with 3'-inverted dT …

为了解决这些问题，我们开发了一种新型生物传感平台，将 CRISPR/Cas12a 系统集成到基于 RCA 的 DNA 水凝胶中。 该平台中使用的水凝胶可以预封装包括 GelRed、亚甲蓝和金纳米颗粒的 …

2023年9月20日 · Rolling circle amplification (RCA) is a widely used DNA amplification method that uses circular template DNA as input and produces multimeric, linear single- or double …

2020年8月25日 · An ingenious and low-cost strategy for constructing large-scale DNA nanoarrays by rolling circle amplification (RCA) and a simplified DNA origami technique is proposed in this …

2024年9月25日 · To address these issues, we developed a novel biosensing platform integrating a CRISPR/Cas12a system into an RCA-based DNA hydrogel. The hydrogel used in the …

2019年9月13日 · 滚环扩增 (RCA) 是一种等温酶促扩增反应，用于大量生产具有预先设计的序列和功能的周期性长单链 DNA/RNA。 RCA技术不仅在构建功能性DNA组装体方面显示出无与伦 …