A ready-to-use mix for SYBR Green qPCR, containing ROX reference and suitable for all qPCR instruments without adjusting the concentration of ROX. 4 x 1.25 ml (500 rxns/20ul reaction)

2021年11月8日 · Quantitative polymerase chain reaction (qPCR) and western blot were used to detect the expression of ACTA2 in the pathological tissues of patients with venous malformations and in control tissues, and the experimental results showed a significantly lower expression of ACTA2 in venous malformation tissues (P < 0.05).

2021年11月7日 · Quantitative polymerase chain reaction (qPCR) and western blot were used to detect the expression of ACTA2 in the pathological tissues of patients with venous malformations and in control tissues, and the experimental results showed a significantly lower expression of ACTA2 in venous malformation tissues (P < 0.05).

2012年3月1日 · Smooth muscle α-actin (Acta2) is one of six highly conserved mammalian actin isoforms that appear to exhibit functional redundancy. Nonetheless, we have postulated a specific functional role for the smooth muscle specific isoform.

进一步的 qPCR 分析表明，ACTA2 的敲低抑制了 Dll4/Notch1 信号通路、Ephrin-B2 信号通路和血管完整性相关分子，并激活了 Hedgehog 信号通路。 结论： 本研究表明，ACTA2缺乏是静脉畸形发病的重要因素，导致血管完整性破坏和血管发育畸形。

Mouse Acta2 qPCR Primer Pair，即小鼠Acta2 qPCR引物对，主要用于基于SYBR Green的qPCR、One-Step qRT-PCR或semi-quantitative PCR。 本引物为预先设计、经过qPCR验证、预混的引物对。

Real-time PCR primer assay designed for SYBR ® Green gene expression analysis. Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

Verified forward and reverse primers for analyzing the quantitative expression of gene. SYBR® Green-based quantitative real-time PCR (qPCR). The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ. Lyophilized qPCR primer mix is shipped at ambiente temperatura.

A ready-to-use mix for SYBR Green qPCR, containing ROX reference and suitable for all qPCR instruments without adjusting the concentration of ROX.

Mouse Acta2 qPCR Primer Pair，即小鼠Acta2 qPCR引物对，主要用于基于SYBR Green的qPCR、One-Step qRT-PCR或semi-quantitative PCR。 本引物为预先设计、经过qPCR验证、预混的引物对。 qPCR (Quantitative PCR)即定量PCR，也称实时荧光定量PCR或实时定量PCR (Real-time quantitative PCR)、实时PCR (Real-time PCR)，是一种在DNA扩增反应过程中，以荧光定量测定每个聚合酶链式反应 (PCR)循环后产物总量的方法。 qPCR常用的两种方法 …