ADCC单抗药物研发之LDH检测 - 知乎
ADCC是指抗体首先与靶细胞表面抗原特异性结合，然后 NK细胞 等具有杀伤细胞功能的细胞通过其表达的Fc受体和包被于靶细胞抗原上的抗体Fc段结合，释放细胞因子和细胞毒性颗粒来杀 …
Evaluation of antibody-dependent cell cytotoxicity using lactate ...
Here, we described a method to evaluate ADCC using a nonradioactive assay based on the measurement of lactate dehydrogenase (LDH) release. NK cells were purified by negative …
LDH检测实验——原理、步骤、注意事项、示例图 - 知乎
乳酸脱氢酶 (lactatedehydrogenase，LDH)是一种广泛存在于各种生物体中稳定的胞浆酶，正常时不能通过细胞膜，当细胞受损伤或死亡时可释放到细胞外，释放出的LDH 在培养基上清中， …
A novel method for evaluating antibody-dependent cell-mediated ...
2016年1月27日 · Analyzing the cytotoxic functions of effector cells, such as NK cells against target cancer cells, is thought to be necessary for predicting the clinical efficacy of antibody …
Development and validation of an antibody-dependent cell …
Antibody-dependent cell-mediated cytotoxicity (ADCC) is an important biological function attributed to the mechanism of action of several therapeutic antibodies, particularly oncology …
细胞杀伤功能及活性检测方法 - 知乎
我们知道在免疫系统中有多种具有杀伤功能的细胞，如 自然杀伤细胞 （NK）、 细胞毒性T细胞 （CTL）、具有 ADCC作用 的相关细胞等。 细胞杀伤是在细胞免疫阶段，作用于靶细胞，使 …
LDH-Glo™ Cytotoxicity Assay | LDH Assay | LDH Release | J2380
The LDH-Glo™ Cytotoxicity assay can be used to assess cell death due to many mechanisms of action of biologics treatments, including complement dependent cytotoxicity (CDC), antibody …
体外药效全视野Vol.1 | 解读抗体药的ADCC检测-金斯瑞生物-科易 …
2021年5月7日 · ADCC是抗体通过与靶细胞表面抗原特异性结合，招募如自然杀伤（Natural killer， NK）细胞等效应细胞，通过非吞噬机制诱导靶细胞死亡的过程。 在抗体介导的ADCC作 …
Antibody-dependent Cell-mediated Cytotoxicity (ADCC) Assay
The lactate dehydrogenase (LDH) approach is another simple and fast ADCC assay. It quantifies the release of LDH, which is a stable cytoplasmic enzyme present in all cells, from cells lysed …
Induction of natural killer antibody-dependent cell cytotoxicity …
2020年9月10日 · To evaluate the potential induction of ADCC by IgG1-isotype mAb treatment, we developed an in vitro LDH cytotoxicity assay by using NK cells, that were derived from PBMCs …