2024年8月1日 · Aldo-keto reductases (AKRs) constitute a superfamily of oxidoreductases that catalyze the reduction of aldehydes and ketones to their corresponding alcohols using NAD (P)H as a coenzyme. Knowledge about the crucial roles of AKRs immobilization in the biocatalytic synthesis of chiral alcohols is expanding.

A non-canonical amino acid (ncAA), p-azido-L-phenylalanine (p-AzF), as the anchor for covalent bonding enzymes, was genetically inserted into preselected sites in the AKR and ADH. Employing the two bioorthogonal counterparts of SpyTag/SpyCatcher and azide–alkyne cycloaddition for the immobilization of AKR and ADH enabled sequential dual ...

2022年4月30日 · In this work, AKR, ADH and GDH mutants containing p-azido-L-phenylalanine (pAzF) were prepared using genetic code expansion, and then AKR was crosslinked using diyne with ADH and GDH in the mixture of cell lysates, respectively. The number and location of pAzF are expected to achieve controllable crosslinked dual enzymes coupled with sellf ...

2024年8月12日 · Zn 2+ 离子诱导最小蛋白质模块的聚集，例如 6-His 标签、富含脯氨酸的基序 （PRM） 和 SRC 同源 3 （SH3） 结构域，并允许偶联的醛酮还原酶 （AKR） 和醇脱氢酶 （ADH） 在低浓度下进行相分离。这是通过将 SpyCatcher 和 PRM-SH3-6His 肽片段分别融合到 AKR 的 C 端和 N 末端 ...

2024年3月8日 · Aldo-keto reductases (AKRs) are a superfamily of oxidoreductases capable of reducing carbonyl to primary and secondary alcohols, found in all phyla 22.

2024年5月27日 · In this study, the SpyTag/SpyCatcher binding pair was fused to the N-terminus of an alcohol dehydrogenase (ADH) and an aldo-keto reductase (AKR), respectively. A non-canonical amino acid (ncAA), p-azido-L-phenylalanine (p-AzF), as the anchor for covalent bonding enzymes, was genetically inserted into preselected sites in the AKR and ADH.

2022年4月30日 · When aldehyde ketone reductase (AKR), alcohol dehydrogenase (ADH) and glucose dehydrogenase (GDH) were utilized as model enzymes, their mutants containing p-azido-L-phenylalanine were bio-orthogonally crosslinked with diyne to form crosslinked dual enzymes (CLDEs) acting as a cascade biological oxidation and reduction system.

Aldo-keto reductases (AKRs) are phase I metabolising enzymes that catalyse the reduced nicotinamide adenine dinucleotide (phosphate) (NAD (P)H)-dependent reduction of carbonyl groups to yield primary and secondary alcohols on a wide range of substrates, including aliphatic and aromatic aldehydes and ketones, ketoprostaglan-dins, ketosteroids and...

2024年3月31日 · Herein, the SpyTag/SpyCatcher binding pair was fused to the N‐terminus of an alcohol dehydrogenase (ADH) and an aldo‐keto reductase (AKR), respectively. A non‐canonical amino acid (ncAA), i.e....

reductase (AKR) and an alcohol dehydrogenase (ADH), respectively, at two preselected sites for each enzyme. The variants were expressed in the genome recoded bacterium .