Question 6 AMP-PNP is a non-hydrolyzable analog of ATP that can bind to proteins in a similar manner as ATP but is no longer hydrolyzed. Predict what would happen to helicase activity if AMP-PNP were added to a DNA replication reaction. Helicase would function normally, since AMP-PNP is an analog of ATP and would function in the place of ATP.

Question: Part 2 point) AMP-PNP is a non-hydrolyzable analog of ATP that can bind to proteins in a similar manner as ATP but is no longer hydrolyzed. Predict what would happen to helicase activity if AMP-PNP were added to a DNA replication reaction Choose one: A Helicase would function normally, since AMP-PNP is an analog of ATP and would ...

AMP-PNP is the abbreviation for a structural analogue of ATP in which the second and. third phosphate groups are linked by an NH group (instead of an oxygen atom). AMP-PNP can bind to the ATP-binding site of most ATPases. It differs from ATP in that the. terminal phosphate cannot be removed by hydrolysis (so you cannot create ADP,

AMP-PNP can bind to the ATP-binding site of most ATPases (enzymes that hydrolyze ATP to ADP + P). AMP-PNP differs from ATP in that the terminal phosphate cannot be removed by hydrolysis (so you AMP-PNP is the abbreviation for a structural analogue of ATP in which the second and third phosphate groups are linked by an NH group (instead of an ...

AMP-PNP can bind to the ATP-binding site of most ATPases. It differs from ATP in that the terminal phosphate cannot be removed by hydrolysis (so you cannot create ADP, therefore, you cannot AMP-PNP is the abbreviation for a structural analogue of ATP in which the second and third phosphate groups are linked by an amino group (instead of an ...

Question: AMP-PNP (shown below) is an inhibitor of ATP synthase when it is operating in the hydrolysis direction (using ATP to generate an H^+ gradient), but not in the synthesis direction (using the H^+ gradient to make ATP from ADP + Pi). a. Mechanistically speaking, why is AMP-PNP an inhibitor of ATP hydrolysis? b.

Because AMP-PNP cannot be converted to ADP by the enzyme, which provides a crystal structure with ATP substrate in the active site. OX-rays destroy ATP because of high energy irradiation, whereas AMP-PNP is more resistant to x-rays …

The ATP analog, AMP-PNP, cannot be hydrolyzed to ADP. What expect would expect this analog to have on filamentous actin if it was bound to actin monomers instead of ATP? Briefly explain your answer.

(5) Kinesins were purified by adding the nonhydrolyzable analog AMP-PNP to cytoplasmic extracts containing microtubules, purifying the microtubules, and then releasing the kinesin proteins, which were still attached to the microtubules, by adding ATP. Would this trick have worked to purify myosin motors attached to actin filaments? Explain.

AMP-PNP (shown below) is an inhibitor of ATP synthase when it is operating in the hydrolysis direction (using ATP to generate an H* gradient), but not in the synthesis direction (using the H gradient to make ATP from ADPP) NH2 O C O-P-N-P-O-P-O HO OH a. b. Considering the mechanism of ATP hydrolysis, why is AMP-PNP an inhibitor?