Single-channel patch-clamp studies using 2F3 cells revealed that atrial natriuretic peptide (ANP) or 8- (4-chlorophenylthio)-cGMP, but not C-type natriuretic peptide or cANP, decreased the open probability of ENaC. This suggests that NPR-A, but not NPR-B or NPR-C, is involved in the natriuretic peptide-mediated regulation of ENaC activity.

2021年5月27日 · In the medullary collecting duct, acting on main cells, ANP reduces epithelial sodium channel (ENaC) opening as well as other sodium channels as cyclic nucleotide-regulated cation channels (CNG; Light et al., 1990, Guo et al., 2013).

Atrial natriuretic peptide (ANP) is a cardiac peptide with multiple physiological effects, including natriuresis, blood pressure regulation, and renin-angiotensin-aldosterone system (RAAS) antagonism. Pre-proANP is synthesized in the atria and must ...

2010年10月1日 · The authors proposed that the defective ANP in the corin knockout mice might result in an overproduction of aldosterone and that this might cause an increase in β-ENaC. They determined the abundance of pathway components (Akt, pSGK1, pAkt, WNK1) that should be upregulated by aldosterone and found them reduced.

2021年8月19日 · ANP and BNP are potent endogenous hypotensive hormones that elicit natriuretic, diuretic, vasorelaxant, antihypertrophic, antiproliferative, and antiinflammatory effects, largely directed toward the reduction of blood pressure (BP) and cardiovascular diseases (CVDs).

2015年5月15日 · The epithelial sodium channel (ENaC) is the main sodium channel for sodium entry and is characterized by low conductance (4–5 pS) with high specificity for Na + over K + (>20-fold), which is inhibited by amiloride (18, 102). Current data on cGMP/PKGII-induced ENaC activation are less conclusive.

2014年4月1日 · The amiloride-sensitive epithelial sodium channel (ENaC) constitutes the rate-limiting step for sodium reabsorption in epithelial cells that line the distal part of the renal tubule, the distal colon, the duct of several exocrine glands, and the lung.

Our patch-clamp single-channel recordings from split-open distal tubule show that SCI decreased the activity of the epithelial sodium channel (ENaC). Western blot combined with confocal microscopy data show that the levels of 70 kD γ-ENaC, which is an active isoform due to proteolytic cleavage, were significantly reduced in distal tubule ...

The data show that SCI significantly reduced the concentrating function of kidney tubules. The reduced water reabsorption appears to be mediated by atrial natriuretic peptide (ANP) because SCI increased the expression levels of both ANP and natriuretic peptide receptor A …

2013年4月1日 · Single-channel patch-clamp studies using 2F3 cells revealed that atrial natriuretic peptide (ANP) or 8- (4-chlorophenylthio)-cGMP, but not C-type natriuretic peptide or cANP, decreased the open probability of ENaC. This suggests that NPR-A, but not NPR-B or NPR-C, is involved in the natriuretic peptide-mediated regulation of ENaC activity.