2018年10月30日 · We demonstrate that expansion of Apj + vessels during tumor development is regulated by hypoxia-VEGF signaling. Finally, our work demonstrates, by genetic cell ablation and using a small peptide Apj antagonist peptide, that Apj + vessels can be targeted to inhibit tumor progression, without affecting vessel integrity in other organs.

2006年8月1日 · A high affinity ligand for APJ, named apelin (APJ endogenous ligand), has been isolated using biochemical techniques (Tatemoto et al., 1998). Apelin mRNA encodes a 77-amino-acid preproprotein that is proteolytically cleaved to yield bioactive peptides 36, 17 and 13 aa in …

Angiotensin II protein J receptor, APJ, is a type A G protein coupled receptor. Endogenous apelin and elabela peptides stimulate APJ via distinct signalling profiles. A complex signalling map of elabela-stimulated APJ was published in 2022. Dimerization or oligomerization of APJ with itself or other receptor(s) can affect APJ signalling.

2025年1月10日 · 为了进一步探究Apelin-APJ系统与VEGF、FGF之间的相互作用机制，我们利用分子生物学技术，如实时荧光定量PCR和蛋白质印迹等方法，检测了Apelin处理后的血管内皮细胞中VEGF和FGF的基因表达和蛋白质水平变化。

2008年7月10日 · To determine the functional significance of hypoxia-induced apelin expression, we knocked down the expression of apelin or its receptor, APJ, by transfecting MEECs with blasticidin expression vectors containing an unrelated miRNA (micontrol), an miRNA directed against apelin (miApelin) or an miRNA directed against APJ (miAPJ) and analyzed their ...

Our analysis clearly showed that APJ expression was induced by VEGF, which, in turn, is well known to be induced by tissue hypoxia (Liu et al, 1995). This indicates that, under tissue hypoxia, blood vessels have an opportunity to enlarge their size and the reduction of APJ expression finalizes the enlargement of blood vessel caliber under ...

Apj+ vessels during tumor development is regulated by hypox-ia-VEGF signaling. Finally, our work demonstrates, by genetic cell ablation and using a small peptide Apj antagonist peptide, that Apj+ vessels can be targeted to inhibit tumor progression, without affecting vessel integrity in other organs. Thus, we iden-

2024年10月3日 · ELA/APJ signaling is known to regulate coronary growth from the SV, whereas VEGF-A/VEGF-R2 signaling controls growth from the endocardium. Previous studies suggest hypoxia might regulate coronary growth, but its specific downstream pathways are unclear.

2018年10月30日 · By genetic targeting using Apj-CreER and Apj-DTRGFP-Luciferase, we demonstrated that hypoxia-VEGF signaling drives expansion of Apj + tumor vessels and that targeting of these vessels, genetically and pharmacologically, remarkably inhibits tumor angiogenesis and restricts tumor growth.

2020年1月21日 · The activation of apelin/APJ signaling significantly decreased the efficacy of VEGF inhibitors in endothelial cells, while disease-free survival (DFS) rates of patients with ovarian cancer treated with bevacizumab (Avastin) significantly decreased with increased expression of apelin, according to a study published in Oncotarget. 1